Esterase (gene EstB) from Alcanivorax dieselolei B-5(T)

Enzyme Description

Species

Alcanivorax dieselolei B-5(T) ↗

Extremophile

No but"cold-adapted" enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 415 amino acids

MAFFPVRRHWLALLGTTVVLTGCAHFSDSDAPPASMEIARGPAGLDFYTPPSPLPEGHHGDLIRARVLENNAALPSAAQNWLTLYRSTDLDGNAIAVSGTVVIPKGTPPEGGWPVISWTHGTTGIADRCAPSRNDDDYPARGYVDLMNETLDQWVRRGYAVVKTDYQGLGTPGVHPYLVGEAEARSAIDMVRAARQLTPDLSRDWLVMGHSQGGQAALYTAYLGPIYAPELNLTGAIAISPASHLSAQIQYALTHPEIRSNPYGALMLVGITAATPKVDLSTLLTEEGRKRIKQVDERCVGEMRGEDGWTLTVAELSDLEADWRPLVQAVASLEDTENLRPSVPLLILQSDDDKAVLKPLTDKLVARYQSLGLDVEYRTYHIENQDQAYTSHQITVPVSRPAAMVWAQRHLPAGR

Shanshan Zhang et al. (2013) — Characterization of EstB, a novel cold-active and organic solvent-tolerant esterase from marine microorganism Alcanivorax dieselolei B-5(T)

Extremophiles · doi:10.1007/s00792-013-0612-y ↗ · Stability - Incubation

32 measurements ▼

Database ID

UniProt: K0C9J9 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethanol	30%	100.0	132.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	1-Butanol	30%	100.0	86.0	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetone	30%	100.0	123.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Isopropanol	30%	100.0	127.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethylene Glycol	30%	100.0	137.7	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetonitrile	10%	100.0	98.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Methanol	10%	100.0	102.0	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	108.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethanol	10%	100.0	119.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	1-Butanol	10%	100.0	105.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetone	10%	100.0	124.1	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Isopropanol	10%	100.0	99.8	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethylene Glycol	50%	100.0	133.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetonitrile	70%	100.0	6.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Methanol	70%	100.0	21.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	70%	100.0	37.7	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethanol	70%	100.0	45.1	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	1-Butanol	70%	100.0	68.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetone	70%	100.0	79.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Isopropanol	70%	100.0	85.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethylene Glycol	70%	100.0	110.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetonitrile	50%	100.0	31.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Methanol	50%	100.0	87.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	83.1	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethanol	50%	100.0	89.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	1-Butanol	50%	100.0	77.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetone	50%	100.0	111.3	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Isopropanol	50%	100.0	93.7	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethylene Glycol	10%	100.0	124.1	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetonitrile	30%	100.0	92.8	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Methanol	30%	100.0	106.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100.0	111.1	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethanol	30%	100.0	132.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	1-Butanol	30%	100.0	86.0	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetone	30%	100.0	123.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Isopropanol	30%	100.0	127.2	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethylene Glycol	30%	100.0	137.7	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetonitrile	10%	100.0	98.5	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Methanol	10%	100.0	102.0	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	108.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Ethanol	10%	100.0	119.9	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	1-Butanol	10%	100.0	105.6	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Acetone	10%	100.0	124.1	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Isopropanol	10%	100.0	99.8	%	Incubation: ?, Assay: 50 mM Tris-HCl, 1% Ethanol	Incubation: ?, Assay: 8.5	Incubation: Room temperature, Assay: 20°C	0.2 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

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Visualization : Stability — Incubation

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