Formate dehydrogenase from Ogataea parapolymorpha DL-1
Enzyme Description
Sequence
MKVVLVLYDAGKHAQDEERLYGCTENALGIRDWLEKQGHELVVTSDKEGENSVLEKNIPDADVIISTPFHPAYITKERIDKAKKLKLLVVAGVGSDHIDLDYINQSGRDISVLEVTGSNVVSVAEHVVMTMLVLVRNFVPAHEQIISGGWNVAEIAKDSFDIEGKVIATIGAGRIGYRVLERLVAFNPKELLYYDYQSLSREAEEKVGARRVHDIKELVAQADIVTINCPLHAGSKGLVNAELLKHFKKGAWLVNTARGAICVAEDVAAAVKSGQLRGYGGDVWYPQPAPKDHPWRSMANKYGAGNAMTPHYSGSVIDAQVRYAQGTKNILESFFTQKFDYRPQDIILLNGKYKTKSYGADK
Shuhuai Yu et al. (2013)
β Promising properties of a formate dehydrogenase from a methanol-assimilating yeast Ogataea parapolymorpha DL-1 in His-tagged form
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-013-4996-5 β
Β· Stability - Incubation
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Database ID
UniParc: UPI0001F75787 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (28 measurements)
28 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 50% | 100.0 | 67.9 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 80% | 100.0 | 2.8 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 80% | 100.0 | 1.2 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | n-Hexane | 80% | 100.0 | 123.7 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | 1-Butanol | 80% | 100.0 | 89.6 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 80% | 100.0 | 2.6 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 80% | 100.0 | 3.4 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 80% | 100.0 | 21.1 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 50% | 100.0 | 5.6 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 119.3 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | n-Hexane | 50% | 100.0 | 109.0 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | 1-Butanol | 50% | 100.0 | 104.4 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 50% | 100.0 | 12.6 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 50% | 100.0 | 51.9 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 10% | 100.0 | 104.2 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 20% | 100.0 | 110.7 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 116.2 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | n-Hexane | 20% | 100.0 | 85.6 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | 1-Butanol | 20% | 100.0 | 91.7 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 20% | 100.0 | 98.4 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer | Incubation: ?, Assay: 6.5 | Incubation: 25Β°C, Assay: 30Β°C | 160 mM Sodium formate | CO2 | 1.6 mM NAD+ | Yes, "incubation with shaking" | Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.