Formate dehydrogenase from Ogataea parapolymorpha DL-1

Enzyme Description

Species
Ogataea parapolymorpha DL-1 β†—
Extremophile
No
EC Number
1.2.1.2 β†—

Sequence

Length: 362 amino acids
MKVVLVLYDAGKHAQDEERLYGCTENALGIRDWLEKQGHELVVTSDKEGENSVLEKNIPDADVIISTPFHPAYITKERIDKAKKLKLLVVAGVGSDHIDLDYINQSGRDISVLEVTGSNVVSVAEHVVMTMLVLVRNFVPAHEQIISGGWNVAEIAKDSFDIEGKVIATIGAGRIGYRVLERLVAFNPKELLYYDYQSLSREAEEKVGARRVHDIKELVAQADIVTINCPLHAGSKGLVNAELLKHFKKGAWLVNTARGAICVAEDVAAAVKSGQLRGYGGDVWYPQPAPKDHPWRSMANKYGAGNAMTPHYSGSVIDAQVRYAQGTKNILESFFTQKFDYRPQDIILLNGKYKTKSYGADK
Shuhuai Yu et al. (2013) β€” Promising properties of a formate dehydrogenase from a methanol-assimilating yeast Ogataea parapolymorpha DL-1 in His-tagged form
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-013-4996-5 β†—  Β· Stability - Incubation
28 measurements ▼
Database ID
UniParc: UPI0001F75787 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (28 measurements)

28 measurements β€” page 2 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Methanol 20% 100.0 90.7 % Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: 30Β°C 160 mM Sodium formate CO2 1.6 mM NAD+ Yes, "incubation with shaking" Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Acetone 20% 100.0 108.1 % Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: 30Β°C 160 mM Sodium formate CO2 1.6 mM NAD+ Yes, "incubation with shaking" Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Isopropanol 10% 100.0 129.3 % Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: 30Β°C 160 mM Sodium formate CO2 1.6 mM NAD+ Yes, "incubation with shaking" Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100.0 111.3 % Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: 30Β°C 160 mM Sodium formate CO2 1.6 mM NAD+ Yes, "incubation with shaking" Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase n-Hexane 10% 100.0 141.2 % Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: 30Β°C 160 mM Sodium formate CO2 1.6 mM NAD+ Yes, "incubation with shaking" Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase 1-Butanol 10% 100.0 93.8 % Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: 30Β°C 160 mM Sodium formate CO2 1.6 mM NAD+ Yes, "incubation with shaking" Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Ethanol 10% 100.0 105.8 % Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: 30Β°C 160 mM Sodium formate CO2 1.6 mM NAD+ Yes, "incubation with shaking" Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Methanol 10% 100.0 95.5 % Incubation: 50 mM sodium phosphate buffer, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 6.5 Incubation: 25Β°C, Assay: 30Β°C 160 mM Sodium formate CO2 1.6 mM NAD+ Yes, "incubation with shaking" Non-incubated control (in %), activity measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent
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Visualization : Stability β€” Incubation

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Structure

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