Lipase rSML from Serratia marcescens ECU101

Enzyme Description

Species
Serratia marcescens ECU1010 β†—
Extremophile
No but "organic solvent-tolerant" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 614 amino acids
MGIFSYKDLDENASKALFSDALAISTYAYHNIDNGFDEGYHQTGFGLGLPLTLVTALIGSTQSQGGLPGLPWNPDSEQAAQEAVNNAGWSVISAAQLGYAGKTDARGTYYGETAGYTTAQAEVLGKYDSEGNLTAIGISFRGTSGPRESLIGDTIGDVINDLLAGFGPKGYADGYTLKAFGNLLGDVAKFAQAHGLSGEDVVVSGHSLGGLAVNSMAAQSDASWSGFYAQSNYVAFASPTQYETGGKVLNIGYENDPVFRALDGTTLTGASLGVHDAPHTSATNNIVNFNDHYASDAWNLLPFSILNIPTWLSHLPFFYQDGLMRVLNSEFYSLTDKDSTIIVSNLSNVTRGNTWVEDLNRNAETHSGPTFIIGSDGNDLIKGGKGNDYLEGRDGDDIFRDAGGYNLIAGGKGHNIFDTQQALKNTEVAYDGNTLYLRDAKGGITLADDISTLRSKETSWLIFNKEVDHQVTAAGLKSDSGLKAYAAAATGGDGDDVLQARSHDAWLFGNAGNDTLIGHAGGNLTFVGGSGDDVLKGVGNGNTFLFSGDFGRDQLYGFNASDKLVFIGTEGASGNIRDYATQQNDDLVLAFGHSQVTLIGVSLDHFSTDQVVLA
Suxia Li et al. (2012) β€” Production, Characterization, and Application of an Organic Solvent-Tolerant Lipase Present in Active Inclusion Bodies
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-012-0028-7 β†—  Β· Stability - Incubation
21 measurements ▼
Database ID
UniProt: Q09KJ5 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (21 measurements)

21 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Acetone 25% 100 251 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Petroleum Ether 50% 100 866 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Diisopropyl Ether 50% 100 1031 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Isopropanol 50% 100 59 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Acetone 50% 100 68 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Ethanol 50% 100 0 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Methanol 50% 100 13 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100 138 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Petroleum Ether 25% 100 677 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Diisopropyl Ether 25% 100 630 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Isopropanol 25% 100 201 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 192 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Ethanol 25% 100 186 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Methanol 25% 100 62 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 100 395 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Petroleum Ether 10% 100 465 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Diisopropyl Ether 10% 100 550 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Isopropanol 10% 100 311 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Acetone 10% 100 298 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Ethanol 10% 100 169 % Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer Incubation: 8, Assay: 7 Incubation: 30Β°C, Assay: 25Β°C 100 mM p-Nitrophenyl acetate Acetic Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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