Lipase rSML from Serratia marcescens ECU101

Enzyme Description

Species

Serratia marcescens ECU1010 ↗

Extremophile

No but "organic solvent-tolerant" enzyme

EC Number

3.1.1.3 ↗

Sequence

Length: 614 amino acids

MGIFSYKDLDENASKALFSDALAISTYAYHNIDNGFDEGYHQTGFGLGLPLTLVTALIGSTQSQGGLPGLPWNPDSEQAAQEAVNNAGWSVISAAQLGYAGKTDARGTYYGETAGYTTAQAEVLGKYDSEGNLTAIGISFRGTSGPRESLIGDTIGDVINDLLAGFGPKGYADGYTLKAFGNLLGDVAKFAQAHGLSGEDVVVSGHSLGGLAVNSMAAQSDASWSGFYAQSNYVAFASPTQYETGGKVLNIGYENDPVFRALDGTTLTGASLGVHDAPHTSATNNIVNFNDHYASDAWNLLPFSILNIPTWLSHLPFFYQDGLMRVLNSEFYSLTDKDSTIIVSNLSNVTRGNTWVEDLNRNAETHSGPTFIIGSDGNDLIKGGKGNDYLEGRDGDDIFRDAGGYNLIAGGKGHNIFDTQQALKNTEVAYDGNTLYLRDAKGGITLADDISTLRSKETSWLIFNKEVDHQVTAAGLKSDSGLKAYAAAATGGDGDDVLQARSHDAWLFGNAGNDTLIGHAGGNLTFVGGSGDDVLKGVGNGNTFLFSGDFGRDQLYGFNASDKLVFIGTEGASGNIRDYATQQNDDLVLAFGHSQVTLIGVSLDHFSTDQVVLA

Suxia Li et al. (2012) — Production, Characterization, and Application of an Organic Solvent-Tolerant Lipase Present in Active Inclusion Bodies

Applied Biochemistry and Biotechnology · doi:10.1007/s12010-012-0028-7 ↗ · Stability - Incubation

21 measurements ▼

Database ID

UniProt: Q09KJ5 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (21 measurements)

21 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase	Methanol	10%	100	101	%	Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer	Incubation: 8, Assay: 7	Incubation: 30°C, Assay: 25°C	100 mM p-Nitrophenyl acetate	Acetic Acid , p-Nitrophenol	—	Incubation: 160 rpm	Non-incubated control (in %), activity measured in aqueous phase \| Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Acetone

25%

100

251

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Petroleum Ether

50%

100

866

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Diisopropyl Ether

50%

100

1031

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Isopropanol

50%

100

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Acetone

50%

100

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Ethanol

50%

100

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Methanol

50%

100

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Dimethyl Sulfoxide (DMSO)

50%

100

138

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Petroleum Ether

25%

100

677

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Diisopropyl Ether

25%

100

630

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Isopropanol

25%

100

201

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10%

100

192

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Ethanol

25%

100

186

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Methanol

25%

100

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Dimethyl Sulfoxide (DMSO)

25%

100

395

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Petroleum Ether

10%

100

465

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Diisopropyl Ether

10%

100

550

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Isopropanol

10%

100

311

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Acetone

10%

100

298

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Ethanol

10%

100

169

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

Stability - Incubation

Activity after incubation (12 hours at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase

Methanol

10%

100

101

Incubation: 50 mM Tris-HCl, Assay: 100 mM potassium buffer

Incubation: 8, Assay: 7

Incubation: 30°C, Assay: 25°C

100 mM p-Nitrophenyl acetate

Acetic Acid, p-Nitrophenol

—

Incubation: 160 rpm

Non-incubated control (in %), activity measured in aqueous phase | Uncertainty about whether the assay was measured with or without organic solvent, uncertainty about control

‹ Prev

1 2

Next ›

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*

Lipase rSML from Serratia marcescens ECU101

Enzyme Description

Sequence

Experimental Data (21 measurements)

Visualization : Stability — Incubation

Structure

Loading…