NAD-specific glutamate dehydrogenase A from Halobacterium salinarum strain NRC-36014
Enzyme Description
Extremophile
Yes
"extremely halophilic" organism
EC Number
Sequence
MTMASKSDSTHDESGDEAADSTEPESALETARRQLYHAASYLDIDQNIVERLKYPKKVHEVTIPIERDDGTVEVFTGYRAQHDSVRGPYKGGLRYHPDVTRDECVGLGMWMTWKCAVMDLPFGGAKGGVAVNPKELSPEEKERLTRRFTQEIRDVIGPNQDIPAPDMGTDPQTMAWLMDAYSMQEGETTPGVVTGKPPVVGGSEGREEAPGRSVAIITQLVCEYYDQPLDETTVAVQGYGSVGANAARLLDKWGATIVAISDVNGAMYEPDGIDTASVPSHDEEPEAVTTYADTVISNEELLTLDVDVLIPAALGNVITKENAEAIAADLVVEGANGPTTSTADSILADRDVAVIPDILANAGGVTVSYFEWLQDINRRAWSLERVNDELEAEMQAAWRAVKDEYENRDVTWRDAAYIVALSRIAEAHEARGLWP
Nayla Munawar et al. (2012)
β Overexpression in a non-native halophilic host and biotechnological potential of NAD+-dependent glutamate dehydrogenase from Halobacterium salinarum strain NRC-36014
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Database ID
UniProt: P29051 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (40 measurements)
40 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Isopropanol | 10% | 100.0 | 33.0 | % | 80 mM glycine-NaOH buffer, 2.6 mM EDTA, 3.2 M NaCl | 8.5 | 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetonitrile | 10% | 100.0 | 30.0 | % | 80 mM glycine-NaOH buffer, 2.6 mM EDTA, 3.2 M NaCl | 8.5 | 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Acetone | 10% | 100.0 | 38.0 | % | 80 mM glycine-NaOH buffer, 2.6 mM EDTA, 3.2 M NaCl | 8.5 | 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Ethanol | 10% | 100.0 | 34.0 | % | 80 mM glycine-NaOH buffer, 2.6 mM EDTA, 3.2 M NaCl | 8.5 | 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Methanol | 10% | 100.0 | 74.0 | % | 80 mM glycine-NaOH buffer, 2.6 mM EDTA, 3.2 M NaCl | 8.5 | 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Glycerol | 10% | 100.0 | 109.0 | % | 80 mM glycine-NaOH buffer, 2.6 mM EDTA, 3.2 M NaCl | 8.5 | 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethylformamide (DMF) | 10% | 100.0 | 51.0 | % | 80 mM glycine-NaOH buffer, 2.6 mM EDTA, 3.2 M NaCl | 8.5 | 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 102.0 | % | 80 mM glycine-NaOH buffer, 2.6 mM EDTA, 3.2 M NaCl | 8.5 | 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Classical aqueous control (in %) |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 10% | 100.0 | 72.3 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 10% | 100.0 | 66.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 10% | 100.0 | 91.3 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 10% | 100.0 | 42.2 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 100.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100.0 | 63.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Glycerol | 10% | 100.0 | 73.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 100.0 | 88.5 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 10% | 100.0 | 40.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 10% | 100.0 | 38.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 10% | 100.0 | 82.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 10% | 100.0 | 6.4 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.