NAD-specific glutamate dehydrogenase A from Halobacterium salinarum strain NRC-36014
Enzyme Description
Extremophile
Yes
"extremely halophilic" organism
EC Number
Sequence
MTMASKSDSTHDESGDEAADSTEPESALETARRQLYHAASYLDIDQNIVERLKYPKKVHEVTIPIERDDGTVEVFTGYRAQHDSVRGPYKGGLRYHPDVTRDECVGLGMWMTWKCAVMDLPFGGAKGGVAVNPKELSPEEKERLTRRFTQEIRDVIGPNQDIPAPDMGTDPQTMAWLMDAYSMQEGETTPGVVTGKPPVVGGSEGREEAPGRSVAIITQLVCEYYDQPLDETTVAVQGYGSVGANAARLLDKWGATIVAISDVNGAMYEPDGIDTASVPSHDEEPEAVTTYADTVISNEELLTLDVDVLIPAALGNVITKENAEAIAADLVVEGANGPTTSTADSILADRDVAVIPDILANAGGVTVSYFEWLQDINRRAWSLERVNDELEAEMQAAWRAVKDEYENRDVTWRDAAYIVALSRIAEAHEARGLWP
Nayla Munawar et al. (2012)
β Overexpression in a non-native halophilic host and biotechnological potential of NAD+-dependent glutamate dehydrogenase from Halobacterium salinarum strain NRC-36014
▼
Database ID
UniProt: P29051 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (40 measurements)
40 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Glycerol | 5% | 100.0 | 87.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 5% | 100.0 | 100.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Glycerol | 5% | 100.0 | 105.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 5% | 100.0 | 100.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 5% | 100.0 | 87.3 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 5% | 100.0 | 89.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 5% | 100.0 | 95.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 5% | 100.0 | 71.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 5% | 100.0 | 103.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 5% | 100.0 | 86.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 5% | 100.0 | 103.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 5% | 100.0 | 96.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 5% | 100.0 | 68.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 5% | 100.0 | 56.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetonitrile | 5% | 100.0 | 95.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (3 days at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 5% | 100.0 | 20.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 104.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100.0 | 94.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Glycerol | 10% | 100.0 | 103.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
| Stability - Incubation | Activity after incubation (0 minute at room temperature), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 100.0 | 96.0 | % | Incubation: 50 mM potassium phosphate buffer, 4M NaCl, Assay: 80 mM glycine-NaOH, 0.9 M NaCl, 2.6 mM EDTA | Incubation: 7.3, Assay: 9 | Incubation: Room temperature, Assay: 40Β°C | 50 mM L-glutamic acid | 2-oxoglutarate | 3 mM NAD+ | β | Water-incubated control (in %), activity in aqueous phase | Clear assay description |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.