Phenylalanine ammonia lyase from Rhodotorula glutinis

Enzyme Description

Species
Rhodotorula glutinis β†—
Extremophile
No
EC Number
4.3.1.5 β†—

Sequence

Length: 706 amino acids
MAPSVDSIATSVANSLSNGLAGDVRKKTSGAGSLLPTTETTQIDIVERILADAGATDQIKLDGYTLTLGDVVGAARHGRTVKVADSPQIREKIDASVEFLRTQLDNSVYGVTTGFGGSADTRTEDAISLQKALLEHQLCGVLPTSMDGFALGRGLENSLPLEVVRGAMTIRVNSLTRGHSAVRIVVLEALTNFLNHGITPIVPLRGTISASGDLSPLSYIAASITGHPDSKVHVDGKIMSAQEAIALKGLQPVVLGPKEGLGLVNGTAVSASMATLALTDAHVLSLLAQANTALTVEAMVGHAGSFHPFLHDVTRPHPTQIEVARNIRTLLEGSKYAVHHETEVKVKDDEGILRQDRYPLRCSPQWLGPLVSDMIHAHSVLSLEAGQSTTDNPLIDLENKMTHHGGAFMASSVGNTMEKTRLAVALMGKVSFTQLTEMLNAGMNRALPSCLAAEDPSLSYHCKGLDIAAAAYTSELGHLANPVSTHVQPAEMGNQAINSLALISARRTAEANDVLSLLLATHLYCVLQAVDLRAMEFEHTKEFEPMVTALLKQHLGALATADVEDKVRKSIYKRLQQNNSYDLEQRWHDTFSVATGAVVEALAGNEVSLASLNAWKVACAEKAIALTRTVRDSFWAAPSSSSPALKYLSPRTRILYSFIREDVGVKARRGDVYLGKQEVTIGTNVSRIYEAIKDGRIAPVLVKMMA
Andrew J. Quinn et al. (2011) β€” Enzyme activity evaluation of organic solvent-treated phenylalanine ammonia lyase
Biotechnology Progress  Β· doi:10.1002/btpr.687 β†—  Β· Stability - Incubation
40 measurements ▼
Database ID
UniProt: A0A3S5G0Z9 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, yeast Rhodotorula glutinis

Experimental Data (40 measurements)

40 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Methanol Β±100% 118.0 110.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Ethanol Β±100% 144.0 131.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Acetonitrile Β±100% 144.0 89.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Acetone Β±100% 144.0 48.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) Β±100% 144.0 120.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase 1-Butanol Β±100% 144.0 27.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase n-Hexane Β±100% 144.0 84.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase n-Heptane Β±100% 144.0 132.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Benzene Β±100% 144.0 29.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Toluene Β±100% 144.0 74.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Methanol Β±100% 144.0 135.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid L-Phenylalanine β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Ethanol Β±100% 118.0 106.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Acetonitrile Β±100% 118.0 75.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Acetone Β±100% 118.0 42.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) Β±100% 118.0 98.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase 1-Butanol Β±100% 118.0 22.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase n-Hexane Β±100% 118.0 71.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase n-Heptane Β±100% 118.0 113.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Benzene Β±100% 118.0 24.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
Stability - Incubation Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (L-phenylalanine absorbance measurement, 520 nm) in aqueous phase Toluene Β±100% 118.0 60.0 U 100 mM Tris-H2SO4, 1.0 M NH4OH assay 9 30Β°C 0.1 M Cinnamic acid methyl ester L-Phenylalanine methyl ester β€” Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

🧬

No structure available for this protein.