Phenylalanine ammonia lyase from Rhodotorula glutinis
Enzyme Description
Sequence
MAPSVDSIATSVANSLSNGLAGDVRKKTSGAGSLLPTTETTQIDIVERILADAGATDQIKLDGYTLTLGDVVGAARHGRTVKVADSPQIREKIDASVEFLRTQLDNSVYGVTTGFGGSADTRTEDAISLQKALLEHQLCGVLPTSMDGFALGRGLENSLPLEVVRGAMTIRVNSLTRGHSAVRIVVLEALTNFLNHGITPIVPLRGTISASGDLSPLSYIAASITGHPDSKVHVDGKIMSAQEAIALKGLQPVVLGPKEGLGLVNGTAVSASMATLALTDAHVLSLLAQANTALTVEAMVGHAGSFHPFLHDVTRPHPTQIEVARNIRTLLEGSKYAVHHETEVKVKDDEGILRQDRYPLRCSPQWLGPLVSDMIHAHSVLSLEAGQSTTDNPLIDLENKMTHHGGAFMASSVGNTMEKTRLAVALMGKVSFTQLTEMLNAGMNRALPSCLAAEDPSLSYHCKGLDIAAAAYTSELGHLANPVSTHVQPAEMGNQAINSLALISARRTAEANDVLSLLLATHLYCVLQAVDLRAMEFEHTKEFEPMVTALLKQHLGALATADVEDKVRKSIYKRLQQNNSYDLEQRWHDTFSVATGAVVEALAGNEVSLASLNAWKVACAEKAIALTRTVRDSFWAAPSSSSPALKYLSPRTRILYSFIREDVGVKARRGDVYLGKQEVTIGTNVSRIYEAIKDGRIAPVLVKMMA
Andrew J. Quinn et al. (2011)
β Enzyme activity evaluation of organic solvent-treated phenylalanine ammonia lyase
Biotechnology Progress
Β· doi:10.1002/btpr.687 β
Β· Stability - Incubation
▼
Database ID
UniProt: A0A3S5G0Z9 β
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, yeast Rhodotorula glutinis
Experimental Data (40 measurements)
40 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Methanol | Β±100% | 4.25 | 3.9 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Ethanol | Β±100% | 4.7 | 4.4 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Acetonitrile | Β±100% | 4.7 | 3.9 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Acetone | Β±100% | 4.7 | 1.5 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | Β±100% | 4.7 | 2.9 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | 1-Butanol | Β±100% | 4.7 | 2.1 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | n-Hexane | Β±100% | 4.7 | 2.9 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | n-Heptane | Β±100% | 4.7 | 4.6 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Benzene | Β±100% | 4.7 | 1.4 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Toluene | Β±100% | 4.7 | 2.6 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Methanol | Β±100% | 4.7 | 4.2 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine | Cinnamic acid | β | 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Ethanol | Β±100% | 4.25 | 4.1 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Acetonitrile | Β±100% | 4.25 | 3.8 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Acetone | Β±100% | 4.25 | 1.5 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | Β±100% | 4.25 | 2.8 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | 1-Butanol | Β±100% | 4.25 | 2.1 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | n-Hexane | Β±100% | 4.25 | 2.7 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | n-Heptane | Β±100% | 4.25 | 4.1 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Benzene | Β±100% | 4.25 | 1.4 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
| Stability - Incubation | Activity after incubation (1 hour) of lyophilized enzyme, measured by absorbance spectrophotometry (Cinnamic acid absorbance measurement, 290 nm) in aqueous phase | Toluene | Β±100% | 4.25 | 2.4 | U | 100 mM Tris-HCl assay | 8.8 | 30Β°C | 37.5 mM L-Phenylalanine methyl ester | Cinnamic acid methyl ester | β | Incubation: ultrasonication (50 Hz/s), Assay: 100 rpm | Water-incubated control (in U) | Bit of uncertainty about protein sequence, lyophilized enzyme incubation |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.
Structure
π§¬
No structure available for this protein.