Ξ²-glucosidase A (gene bglA) from Caldicellulosiruptor saccharolyticus
Enzyme Description
Extremophile
Yes
"thermophilic" organism
EC Number
Sequence
MDMSFPKGFLWGAATASYQIEGAWNEDGKGESIWDRFTHQKRNILYGHNGDVACDHYHRFEEDVSLMKELGLKAYRFSIAWTRIFPDGFGTVNQKGLEFYDRLINKLVENGIEPVVTLYHWDLPQKLQDIGGWANPEIVNYYFDYAMLVINRYKDKVKKWITFNEPYCIAFLGYFHGIHAPGIKDFKVAMDVVHSLMLSHFKVVKAVKENNIDVEVGITLNLTPVYLQTERLGYKVSEIEREMVSLSSQLDNQLFLDPVLKGSYPQKLLDYLVQKDLLDSQKALSMQQEVKENFIFPDFLGINYYTRAVRLYDENSSWIFPIRWEHPAGEYTEMGWEVFPQGLFDLLIWIKESYPQIPIYITENGAAYNDIVTEDGKVHDSKRIEYLKQHFEAARKAIENGVDLRGYFVWSLMDNFEWAMGYTKRFGIIYVDYETQKRIKKDSFYFYQQYIKENS
Adrian R. Plant et al. (1988)
β Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli
Archives of Biochemistry and Biophysics
Β· doi:10.1016/0003-9861(88)90180-4 β
Β· Stability - Incubation
▼
Database ID
UniProt: P10482 β
Sequence Annotation
Inferred - from protein name
Protein Source
Recombinant, host bacterium Escherichia coli strain PB2481
Experimental Data (28 measurements)
28 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 20% | 100.0 | 99.2 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Tetrahydrofuran (THF) | 90% | 100.0 | 0.0 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Tetrahydrofuran (THF) | 80% | 100.0 | 0.0 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Tetrahydrofuran (THF) | 60% | 100.0 | 4.8 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Tetrahydrofuran (THF) | 50% | 100.0 | 14.4 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Tetrahydrofuran (THF) | 40% | 100.0 | 30.3 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Tetrahydrofuran (THF) | 30% | 100.0 | 73.9 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Tetrahydrofuran (THF) | 20% | 100.0 | 107.2 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Tetrahydrofuran (THF) | 10% | 100.0 | 108.8 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 90% | 100.0 | 0.0 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 80% | 100.0 | 0.0 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 60% | 100.0 | 9.6 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 50% | 100.0 | 107.7 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Methanol | 40% | 100.0 | 103.4 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Ethanol | 10% | 100.0 | 112.2 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 95% | 100.0 | 58.0 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 75% | 100.0 | 95.5 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 60% | 100.0 | 104.0 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 50% | 100.0 | 111.2 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
| Stability - Incubation | Activity after incubation (10 minutes at 50Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase | Acetone | 40% | 100.0 | 110.1 | % | Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM Ξ²-mercaptoethanol, 0.5 MM EDTA | Incubation: 6.3, Assay: 6.3 | Incubation: 50Β°C, Assay: 70Β°C | 10 mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay conditions |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.