β-glucosidase A (gene bglA) from Caldicellulosiruptor saccharolyticus

Enzyme Description

Species

Caldicellulosiruptor saccharolyticus ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.2.1.21 ↗

Sequence

Length: 455 amino acids

MDMSFPKGFLWGAATASYQIEGAWNEDGKGESIWDRFTHQKRNILYGHNGDVACDHYHRFEEDVSLMKELGLKAYRFSIAWTRIFPDGFGTVNQKGLEFYDRLINKLVENGIEPVVTLYHWDLPQKLQDIGGWANPEIVNYYFDYAMLVINRYKDKVKKWITFNEPYCIAFLGYFHGIHAPGIKDFKVAMDVVHSLMLSHFKVVKAVKENNIDVEVGITLNLTPVYLQTERLGYKVSEIEREMVSLSSQLDNQLFLDPVLKGSYPQKLLDYLVQKDLLDSQKALSMQQEVKENFIFPDFLGINYYTRAVRLYDENSSWIFPIRWEHPAGEYTEMGWEVFPQGLFDLLIWIKESYPQIPIYITENGAAYNDIVTEDGKVHDSKRIEYLKQHFEAARKAIENGVDLRGYFVWSLMDNFEWAMGYTKRFGIIYVDYETQKRIKKDSFYFYQQYIKENS

Adrian R. Plant et al. (1988) — Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli

Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(88)90180-4 ↗ · Stability - Incubation

28 measurements ▼

Database ID

UniProt: P10482 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host bacterium Escherichia coli strain PB2481

Experimental Data (28 measurements)

28 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	30%	100.0	112.2	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	106.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	90%	100.0	63.8	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	80%	100.0	87.0	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	67%	100.0	93.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100.0	97.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	110.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	30%	100.0	118.6	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions

Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	20%	100.0	99.2	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Tetrahydrofuran (THF)	90%	100.0	0.0	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Tetrahydrofuran (THF)	80%	100.0	0.0	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Tetrahydrofuran (THF)	60%	100.0	4.8	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Tetrahydrofuran (THF)	50%	100.0	14.4	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Tetrahydrofuran (THF)	40%	100.0	30.3	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Tetrahydrofuran (THF)	30%	100.0	73.9	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Tetrahydrofuran (THF)	20%	100.0	107.2	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Tetrahydrofuran (THF)	10%	100.0	108.8	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	90%	100.0	0.0	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	80%	100.0	0.0	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	60%	100.0	9.6	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	107.7	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Methanol	40%	100.0	103.4	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	112.2	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	95%	100.0	58.0	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	75%	100.0	95.5	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	60%	100.0	104.0	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	111.2	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	40%	100.0	110.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	30%	100.0	112.2	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	106.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	90%	100.0	63.8	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	80%	100.0	87.0	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	67%	100.0	93.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	60%	100.0	97.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	110.1	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions
Stability - Incubation	Activity after incubation (10 minutes at 50°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase	Ethanol	30%	100.0	118.6	%	Incubation: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA, Assay: 100 mM 3-[N-morpholino]propanesulfonic acid, 1 mM β-mercaptoethanol, 0.5 MM EDTA	Incubation: 6.3, Assay: 6.3	Incubation: 50°C, Assay: 70°C	10 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay conditions

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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