NAD+-dependent formate dehydrogenase from Bacillus sp. F1
Enzyme Description
Sequence
MAKIVCVLYDDPVTGYPKTYARDDLPKIECYPDGQTLPTPRAIDFQPGALLGSVSGELGLRKYLESNGHELVVTSSKDGDNSVLDRELADAEIVISQPFWPAYMTAERIKRAKKLKMIVTAGIGSDHTDLQAAMEHGITVAEVTYCNSNSVAEHVMMTTLALVRNYLPSYQWVLKGGWNIADCVERSYDLEGMHVGTVAAGRIGLRVLRLMKPFGTHLHYLDRHRLPESVEKELNLTHHTSLESLAKVCDVVTLNCPLHPETEHMINADSLKHFKRGAYLINTARGKLCDRDAVAAALESGQLAGYGGDVWFPQPAPADHPWRSMPHHGMTPHISGTSLSAQTRYAAGTREILECYFENRPIRNEYLIVQNGKLAGVGAHSYSAGNATGGSEEAARFKKSA
H.-T. Ding et al. (2011)
β Characterization of a thermally stable and organic solvent-adaptative NAD+-dependent formate dehydrogenase from Bacillus sp. F1
Journal of Applied Microbiology
Β· doi:10.1111/j.1365-2672.2011.05124.x β
Β· Stability - Incubation
▼
Database ID
UniProt: D6CJK0 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (40 measurements)
40 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isoamyl Alcohol | 60% | 100.0 | 86.78 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | 1-Butanol | 50% | 100.0 | 94.23 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isoamyl Alcohol | 50% | 100.0 | 74.22 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | n-Hexane | 50% | 100.0 | 8.39 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 60% | 100.0 | 0.0 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 60% | 100.0 | 102.23 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 60% | 100.0 | 0.0 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 60% | 100.0 | 36.71 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 60% | 100.0 | 101.49 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | 1-Butanol | 60% | 100.0 | 141.21 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 50% | 100.0 | 98.1 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | n-Hexane | 60% | 100.0 | 0.0 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 80% | 100.0 | 0.0 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 80% | 100.0 | 46.33 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 80% | 100.0 | 0.0 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 80% | 100.0 | 15.44 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 80% | 100.0 | 22.8 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | 1-Butanol | 80% | 100.0 | 91.2 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isoamyl Alcohol | 80% | 100.0 | 0.0 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | n-Hexane | 80% | 100.0 | 0.0 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.