NAD+-dependent formate dehydrogenase from Bacillus sp. F1
Enzyme Description
Sequence
MAKIVCVLYDDPVTGYPKTYARDDLPKIECYPDGQTLPTPRAIDFQPGALLGSVSGELGLRKYLESNGHELVVTSSKDGDNSVLDRELADAEIVISQPFWPAYMTAERIKRAKKLKMIVTAGIGSDHTDLQAAMEHGITVAEVTYCNSNSVAEHVMMTTLALVRNYLPSYQWVLKGGWNIADCVERSYDLEGMHVGTVAAGRIGLRVLRLMKPFGTHLHYLDRHRLPESVEKELNLTHHTSLESLAKVCDVVTLNCPLHPETEHMINADSLKHFKRGAYLINTARGKLCDRDAVAAALESGQLAGYGGDVWFPQPAPADHPWRSMPHHGMTPHISGTSLSAQTRYAAGTREILECYFENRPIRNEYLIVQNGKLAGVGAHSYSAGNATGGSEEAARFKKSA
H.-T. Ding et al. (2011)
β Characterization of a thermally stable and organic solvent-adaptative NAD+-dependent formate dehydrogenase from Bacillus sp. F1
Journal of Applied Microbiology
Β· doi:10.1111/j.1365-2672.2011.05124.x β
Β· Stability - Incubation
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Database ID
UniProt: D6CJK0 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (40 measurements)
40 measurements β page 2 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 20% | 100.0 | 76.8 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 10% | 100.0 | 89.3 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 10% | 100.0 | 97.04 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 10% | 100.0 | 112.52 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 10% | 100.0 | 84.54 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | 1-Butanol | 10% | 100.0 | 100.61 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isoamyl Alcohol | 10% | 100.0 | 91.68 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | n-Hexane | 10% | 100.0 | 100.01 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 99.39 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 20% | 100.0 | 105.85 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 95.25 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 20% | 100.0 | 132.31 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isopropanol | 20% | 100.0 | 98.75 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | 1-Butanol | 20% | 100.0 | 74.87 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Isoamyl Alcohol | 20% | 100.0 | 80.03 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | n-Hexane | 20% | 100.0 | 119.4 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 60.02 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Acetone | 50% | 100.0 | 104.56 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Methanol | 50% | 100.0 | 7.74 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
| Stability - Incubation | Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase | Ethanol | 50% | 100.0 | 72.28 | % | Incubation: 50 mM sodium phosphate buffer, 500 mM NaCl, Assay: 100 mM Tris-HCl | Incubation: 8, Assay: 7 | Incubation: 25Β°C, Assay: 25Β°C | 100 mM Sodium formate | CO2 | 1 mM NAD+ | β | Non-incubated control (in %), residual activity measured in aqueous phase | High uncertainty about whether activity measured with or without organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.