Family VII esterase from a compost metagenomic library
Enzyme Description
Sequence
MMRAELHRSPRQHRLGKQRQHEEDDPVRTKFGAWKPLTALASAILLAASVAGCGSSSKSDDREPPPAPRMGNYQGTDEGSMLAFRGIRYAKPPVGELRFAPPQPVEKPTGIVVADKFGSACPQYQSAFGPESLEEDCLFLNVYVPKAGEGPFPVMVWIHGGAFVAGSGGPEYDPSRLVAQGVVVVAINYRLGPLGFLAHPDLTNEQGGGSGNYGLMDQQLALRWVQDNISTFGGDPNNVTIFGESAGGLSVLSHVVSPKAQGLFQRAIVQSGSYDAISRTLAEAEQAGQDFVAAVGCDSAGDIPQCLRELDVKTILDNGDAIVAGLSLTPHLHPDLLPKSINQALQEGKVNVNSILLGSNRDEFTLFIALEELNGTPPMEEDDVEDQWSKFFDPLTSIVSTYYPATDFENPSRRFAAPLGDSVFSCTALRQAEQLGSTIPVYVYEFADRDAPSILPKVSFDLGAAHAFEIQYVLGSEQALRARGANDSQIALSNAMVQYWTAFARTGDPNQGEAEAIWPEFGSNNGNIRWLNPAEPEKTVITSAEFEADHRCEIWDKIIFQALKQDPQKS
Chul-Hyung Kang et al. (2011)
β A novel family VII esterase with industrial potential from compost metagenomic library
Microbial Cell Factories
Β· doi:10.1186/1475-2859-10-41 β
Β· Activity + Stability - Incubation
▼
Database ID
NCBI: GU256649 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (24 measurements)
24 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 20% | 100.0 | 92.1 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50% | 100.0 | 50.9 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 50% | 100.0 | 3.3 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 97.4 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 50% | 100.0 | 4.4 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 1-Butanol | 50% | 100.0 | 2.4 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 50% | 100.0 | 4.7 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 50% | 100.0 | 8.7 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 50% | 100.0 | 12.6 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 115.1 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 20% | 100.0 | 15.6 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 128.2 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 119.9 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 1-Butanol | 20% | 100.0 | 98.2 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 20% | 100.0 | 131.3 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 138.2 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 101.4 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 110.3 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 10% | 100.0 | 44.8 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 125.9 | % | Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol | Incubation: 8, Assay: 9 | Incubation: 30Β°C, Assay: 25Β°C | 10 mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Water-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.