Family VII esterase from a compost metagenomic library

Enzyme Description

Species

Na (compost metagenomic library)

Extremophile

EC Number

3.1.1.1 ↗

Sequence

Length: 570 amino acids

MMRAELHRSPRQHRLGKQRQHEEDDPVRTKFGAWKPLTALASAILLAASVAGCGSSSKSDDREPPPAPRMGNYQGTDEGSMLAFRGIRYAKPPVGELRFAPPQPVEKPTGIVVADKFGSACPQYQSAFGPESLEEDCLFLNVYVPKAGEGPFPVMVWIHGGAFVAGSGGPEYDPSRLVAQGVVVVAINYRLGPLGFLAHPDLTNEQGGGSGNYGLMDQQLALRWVQDNISTFGGDPNNVTIFGESAGGLSVLSHVVSPKAQGLFQRAIVQSGSYDAISRTLAEAEQAGQDFVAAVGCDSAGDIPQCLRELDVKTILDNGDAIVAGLSLTPHLHPDLLPKSINQALQEGKVNVNSILLGSNRDEFTLFIALEELNGTPPMEEDDVEDQWSKFFDPLTSIVSTYYPATDFENPSRRFAAPLGDSVFSCTALRQAEQLGSTIPVYVYEFADRDAPSILPKVSFDLGAAHAFEIQYVLGSEQALRARGANDSQIALSNAMVQYWTAFARTGDPNQGEAEAIWPEFGSNNGNIRWLNPAEPEKTVITSAEFEADHRCEIWDKIIFQALKQDPQKS

Chul-Hyung Kang et al. (2011) — A novel family VII esterase with industrial potential from compost metagenomic library

Microbial Cell Factories · doi:10.1186/1475-2859-10-41 ↗ · Activity + Stability - Incubation

24 measurements ▼

Database ID

NCBI: GU256649 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	118.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	10%	100.0	99.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100.0	132.5	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	117.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	92.1	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100.0	50.9	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	50%	100.0	3.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	97.4	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	50%	100.0	4.4	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	50%	100.0	2.4	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	50%	100.0	4.7	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	8.7	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	50%	100.0	12.6	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100.0	115.1	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	100.0	15.6	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	128.2	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100.0	119.9	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	20%	100.0	98.2	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	100.0	131.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100.0	138.2	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100.0	101.4	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100.0	110.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100.0	44.8	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	125.9	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	118.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	10%	100.0	99.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100.0	132.5	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	117.3	%	Incubation: 100 mM GTA buffer, 4% Ethanol assay, Assay: 100 mM GTA buffer, 4% Ethanol	Incubation: 8, Assay: 9	Incubation: 30°C, Assay: 25°C	10 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent

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Visualization : Activity + Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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