Lipase from Geobacillus sp. ARM
Enzyme Description
Sequence
MMKCCRRVALVLLGLWLVFCLSVPGGQAEAAASRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQLLNAQGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRVHIIAHSQGGQTARMLVSLLENGSKEEREYAKAHNVSLSPLFEGGHNFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLKAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDQYFKRLKQSPVWTSADTARYDLSVPGAEALNQWVQASPNTYYLSFATERTYRGALTGNYYPELGMNVFSAAVCAPFLGSYRNAALGIDDRWLENDGIVNTFSMNGPKRGSTDRIVPYDGTLKKGVWNDMGTYNVDHLEVIGVDPNPLFDIHAFYLRLAEQLASLQP
Afshin Ebrahimpour et al. (2011)
β High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM
Bioresource Technology
Β· doi:10.1016/j.biortech.2011.03.083 β
Β· Activity + Stability - Incubation
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Database ID
UniProt: A0A0D8BQX7 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli TOP10
Experimental Data (27 measurements)
27 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Toluene | 30% | 100 | 165 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 62 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | 2,3-Butanediol | 30% | 100 | 100 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Methanol | 30% | 100 | 107 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Acetonitrile | 30% | 100 | 1 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Ethanol | 30% | 100 | 116 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Acetone | 30% | 100 | 2 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Isopropanol | 30% | 100 | 3 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | 1-Propanol | 30% | 100 | 1 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Isobutanol | 30% | 100 | 4 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | 1-Butanol | 30% | 100 | 1 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Benzyl Alcohol | 30% | 100 | 0 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Isoamyl Alcohol | 30% | 100 | 21 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Benzene | 30% | 100 | 142 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | n-Hexadecane | 30% | 100 | 77 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | 1-Octanol | 30% | 100 | 137 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | o-Xylene | 30% | 100 | 136 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | Ethylbenzene | 30% | 100 | 77 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | n-Hexane | 30% | 100 | 139 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetateβpyridine reagent product, 715 nm) in the presence of organic solvent | 1-Decanol | 30% | 100 | 86 | % | Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl | Incubation: 7, Assay: 8 | Incubation: 30Β°C, Assay: 65Β°C | emulsion : 50% v/v Olive oil | free fatty acids , glycerol | β | Incubation: 150 rpm | Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent |
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.