Lipase from Geobacillus sp. ARM

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 418 amino acids

MMKCCRRVALVLLGLWLVFCLSVPGGQAEAAASRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQLLNAQGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRVHIIAHSQGGQTARMLVSLLENGSKEEREYAKAHNVSLSPLFEGGHNFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLKAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDQYFKRLKQSPVWTSADTARYDLSVPGAEALNQWVQASPNTYYLSFATERTYRGALTGNYYPELGMNVFSAAVCAPFLGSYRNAALGIDDRWLENDGIVNTFSMNGPKRGSTDRIVPYDGTLKKGVWNDMGTYNVDHLEVIGVDPNPLFDIHAFYLRLAEQLASLQP

Afshin Ebrahimpour et al. (2011) — High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM

Bioresource Technology · doi:10.1016/j.biortech.2011.03.083 ↗ · Activity + Stability - Incubation

27 measurements ▼

Database ID

UniProt: A0A0D8BQX7 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli TOP10

Experimental Data (27 measurements)

27 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	n-Heptane	30%	100	150	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl	Incubation: 7, Assay: 8	Incubation: 30°C, Assay: 65°C	emulsion : 50% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	Isooctane	30%	100	128	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl	Incubation: 7, Assay: 8	Incubation: 30°C, Assay: 65°C	emulsion : 50% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	1-Undecanol	30%	100	74	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl	Incubation: 7, Assay: 8	Incubation: 30°C, Assay: 65°C	emulsion : 50% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	1-Dodecanol	30%	100	150	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl	Incubation: 7, Assay: 8	Incubation: 30°C, Assay: 65°C	emulsion : 50% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	n-Tetradecane	30%	100	116	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl	Incubation: 7, Assay: 8	Incubation: 30°C, Assay: 65°C	emulsion : 50% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	n-Pentadecane	30%	100	128	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl	Incubation: 7, Assay: 8	Incubation: 30°C, Assay: 65°C	emulsion : 50% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent
Activity + Stability - Incubation	Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent	n-Heptadecane	30%	100	142	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl	Incubation: 7, Assay: 8	Incubation: 30°C, Assay: 65°C	emulsion : 50% v/v Olive oil	free fatty acids , glycerol	—	Incubation: 150 rpm	Non-incubated control (in %), activity measured in the presence of organic solvent \| Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Activity after incubation (30 minutes at 30°C), measured by absorbance spectrophotometry (colorimetric Kwon-Rhee assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in the presence of organic solvent

Toluene

30%

100

165

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Dimethyl Sulfoxide (DMSO)

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

2,3-Butanediol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Methanol

30%

100

107

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Acetonitrile

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Ethanol

30%

100

116

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Acetone

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Isopropanol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

1-Propanol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Isobutanol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

1-Butanol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Benzyl Alcohol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Isoamyl Alcohol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Benzene

30%

100

142

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

n-Hexadecane

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

1-Octanol

30%

100

137

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

o-Xylene

30%

100

136

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Ethylbenzene

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

n-Hexane

30%

100

139

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

1-Decanol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

n-Heptane

30%

100

150

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

Isooctane

30%

100

128

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

1-Undecanol

30%

100

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

1-Dodecanol

30%

100

150

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

n-Tetradecane

30%

100

116

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

n-Pentadecane

30%

100

128

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

Activity + Stability - Incubation

n-Heptadecane

30%

100

142

Incubation: 50 mM phosphate buffer, Assay: 50 mM Tris-HCl

Incubation: 7, Assay: 8

Incubation: 30°C, Assay: 65°C

emulsion : 50% v/v Olive oil

free fatty acids, glycerol

—

Incubation: 150 rpm

Non-incubated control (in %), activity measured in the presence of organic solvent | Uncertainty about whether assay done with or without organic solvent

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Visualization : Activity + Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Lipase from Geobacillus sp. ARM

Enzyme Description

Sequence

Experimental Data (27 measurements)

Visualization : Activity + Stability — Incubation

Structure

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