Lipase (gene lip9) from Pseudomonas aeruginosa LST-03

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 311 amino acids

MKKKSLLPLGLAIGLASLAASPLIQASTYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAILSGLVNSLGALISFLSSGSTGTQNSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKVNGVSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLTSLFETSPVSVYRQHANRLKNASL

Hiroyasu Ogino et al. (2000) — Purification and characterization of organic solvent-stable lipase from organic solvent-tolerant Pseudomonas aeruginosa LST-03

Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(00)89095-7 ↗ · Activity - Classical Stability - Half-life

43 measurements ▼

Database ID

UniProt: A8QYB2 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Pseudomonas aeruginosa LST-03

Experimental Data (43 measurements)

43 measurements — page 3 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Half-life	Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)	Dimethyl Sulfoxide (DMSO)	25%	12.5	36.2	days	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM Dimercaptopropan-1-ol tributyrate	Butyric Acid , Free thiols	—	Incubation: 160 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)	Ethylene Glycol	25%	12.5	50.0	days	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM Dimercaptopropan-1-ol tributyrate	Butyric Acid , Free thiols	—	Incubation: 160 rpm	Classical aqueous control (in days)
Stability - Half-life	Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)	n-Decane	25%	12.5	>100.0	days	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM Dimercaptopropan-1-ol tributyrate	Butyric Acid , Free thiols	—	Incubation: 160 rpm	Classical aqueous control (in days)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Octane

50%

1.0

1.01

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Chloroform

50%

1.0

0.1

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Hexanol

50%

1.0

0.11

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Pentanol

50%

1.0

0.12

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Heptanol

50%

1.0

0.13

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Decanol

50%

1.0

0.14

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Octanol

50%

1.0

0.15

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

1-Butanol

50%

1.0

0.16

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Benzene

50%

1.0

0.19

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Ethanol

50%

1.0

0.21

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Toluene

50%

1.0

0.23

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Methanol

50%

1.0

0.25

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Isopropanol

50%

1.0

0.28

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

p-Xylene

50%

1.0

0.46

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Acetone

50%

1.0

0.35

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Decane

50%

1.0

1.1

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

1.0

0.99

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Dimethylformamide (DMF)

50%

1.0

0.92

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Heptane

50%

1.0

0.84

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Dodecane

50%

1.0

0.73

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Cyclohexane

50%

1.0

0.71

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

Isooctane

50%

1.0

0.65

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Hexane

50%

1.0

0.57

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Hexadecane

50%

1.0

0.57

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Activity - Classical

Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent

n-Tetradecane

50%

1.0

0.47

relative units

50 mM McIlvain buffer

30°C

2g/20 ml (including organic solvent) Tributyrin

Butyric Acid, glycerol

—

700 rpm

Classical aqueous control (in relative units)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

tert-Butanol

25%

12.5

1.2

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

1,5-Pentanediol

25%

12.5

6.8

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Ethanol

25%

12.5

7.6

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Triethylene Glycol

25%

12.5

7.7

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

p-Xylene

25%

12.5

8.3

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Toluene

25%

12.5

9.0

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

1,4-Butanediol

25%

12.5

9.3

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

12.5

9.5

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Glycerol

25%

12.5

10.8

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Methanol

25%

12.5

11.3

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Hexane

25%

12.5

11.6

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Cyclohexane

25%

12.5

15.7

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Isooctane

25%

12.5

16.4

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Heptane

25%

12.5

17.7

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Octane

25%

12.5

29.0

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

12.5

36.2

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Ethylene Glycol

25%

12.5

50.0

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Decane

25%

12.5

>100.0

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM Dimercaptopropan-1-ol tributyrate

Butyric Acid, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

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Visualization : Activity — Classical

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Visualization : Stability — Half-life

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Lipase (gene lip9) from Pseudomonas aeruginosa LST-03

Enzyme Description

Sequence

Experimental Data (43 measurements)

Visualization : Activity — Classical

Visualization : Stability — Half-life

Structure

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