Lipase (gene lip9) from Pseudomonas aeruginosa LST-03

Enzyme Description

Species
Pseudomonas aeruginosa LST-03 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 311 amino acids
MKKKSLLPLGLAIGLASLAASPLIQASTYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAILSGLVNSLGALISFLSSGSTGTQNSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKVNGVSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLTSLFETSPVSVYRQHANRLKNASL
Hiroyasu Ogino et al. (2000) β€” Purification and characterization of organic solvent-stable lipase from organic solvent-tolerant Pseudomonas aeruginosa LST-03
Journal of Bioscience and Bioengineering  Β· doi:10.1016/s1389-1723(00)89095-7 β†—  Β· Activity - Classical Stability - Half-life
43 measurements ▼
Database ID
UniProt: A8QYB2 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Pseudomonas aeruginosa LST-03

Experimental Data (43 measurements)

43 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent Cyclohexane 50% 1.0 0.71 relative units 50 mM McIlvain buffer 7 30Β°C 2g/20 ml (including organic solvent) Tributyrin Butyric Acid , glycerol β€” 700 rpm Classical aqueous control (in relative units)
Activity - Classical Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent Isooctane 50% 1.0 0.65 relative units 50 mM McIlvain buffer 7 30Β°C 2g/20 ml (including organic solvent) Tributyrin Butyric Acid , glycerol β€” 700 rpm Classical aqueous control (in relative units)
Activity - Classical Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent n-Hexane 50% 1.0 0.57 relative units 50 mM McIlvain buffer 7 30Β°C 2g/20 ml (including organic solvent) Tributyrin Butyric Acid , glycerol β€” 700 rpm Classical aqueous control (in relative units)
Activity - Classical Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent n-Hexadecane 50% 1.0 0.57 relative units 50 mM McIlvain buffer 7 30Β°C 2g/20 ml (including organic solvent) Tributyrin Butyric Acid , glycerol β€” 700 rpm Classical aqueous control (in relative units)
Activity - Classical Relative activity, measured by pH-metry (free fatty acid titration with NaOH) in the presence of organic solvent n-Tetradecane 50% 1.0 0.47 relative units 50 mM McIlvain buffer 7 30Β°C 2g/20 ml (including organic solvent) Tributyrin Butyric Acid , glycerol β€” 700 rpm Classical aqueous control (in relative units)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) tert-Butanol 25% 12.5 1.2 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) 1,5-Pentanediol 25% 12.5 6.8 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) Ethanol 25% 12.5 7.6 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) Triethylene Glycol 25% 12.5 7.7 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) p-Xylene 25% 12.5 8.3 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) Toluene 25% 12.5 9.0 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) 1,4-Butanediol 25% 12.5 9.3 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) Dimethylformamide (DMF) 25% 12.5 9.5 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) Glycerol 25% 12.5 10.8 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) Methanol 25% 12.5 11.3 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) n-Hexane 25% 12.5 11.6 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) Cyclohexane 25% 12.5 15.7 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) Isooctane 25% 12.5 16.4 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) n-Heptane 25% 12.5 17.7 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
Stability - Half-life Half-life (in days) with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) n-Octane 25% 12.5 29.0 days Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl Incubation: 8, Assay: 8 Incubation: 30Β°C, Assay: 30Β°C 2 mM Dimercaptopropan-1-ol tributyrate Butyric Acid , Free thiols β€” Incubation: 160 rpm Classical aqueous control (in days)
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Visualization : Activity β€” Classical

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Visualization : Stability β€” Half-life

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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