Alcohol dehydrogenase from Aeropyrum pernix

Enzyme Description

Species

Aeropyrum pernix ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

1.1.1.1 ↗

Sequence

Length: 344 amino acids

MKAARLHEYNKPLRIEDVDYPRLEGRFDVIVRIAGAGVCHTDLHLVQGMWHELLQPKLPYTLGHENVGYIEEVAEGVEGLEKGDPVILHPAVTDGTCLACRAGEDMHCENLEFPGLNIDGGFAEFMRTSHRSVIKLPKDISREKLVEMAPLADAGITAYRAVKKAARTLYPGAYVAIVGVGGLGHIAVQLLKVMTPATVIALDVKEEKLKLAERLGADHVVDARRDPVKQVMELTRGRGVNVAMDFVGSQATVDYTPYLLGRMGRLIIVGYGGELRFPTIRVISSEVSFEGSLVGNYVELHELVTLALQGKVRVEVDIHKLDEINDVLERLEKGEVLGRAVLIP

Hidehiko Hirakawa et al. (2005) — Log P effect of organic solvents on a thermophilic alcohol dehydrogenase

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2004.12.007 ↗ · Activity - Michaelis-Menten

24 measurements ▼

Database ID

UniProt: Q9Y9P9 ↗

Sequence Annotation

Inferred

Protein Source

Purified, archaeon Aeropyrum pernix

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Michaelis-Menten	kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethylformamide (DMF)	0.05 (molar fraction)	0.12	0.38	1/s	100 mM potassium phosphate	8	60°C	1-Pentanol	Pentanal	0.1 mM NAD+	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.2 (molar fraction)	0.12	0.28	1/s	100 mM potassium phosphate	8	60°C	1-Pentanol	Pentanal	0.1 mM NAD+	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.15 (molar fraction)	0.12	0.28	1/s	100 mM potassium phosphate	8	60°C	1-Pentanol	Pentanal	0.1 mM NAD+	—	Classical aqueous control (in 1/s)
Activity - Michaelis-Menten	kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	0.1 (molar fraction)	0.12	0.18	1/s	100 mM potassium phosphate	8	60°C	1-Pentanol	Pentanal	0.1 mM NAD+	—	Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.05 (molar fraction)

0.33

0.28

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Acetonitrile

0.2 (molar fraction)

0.33

26.8

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Acetonitrile

0.15 (molar fraction)

0.33

26.9

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Acetonitrile

0.1 (molar fraction)

0.33

28.9

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Acetonitrile

0.05 (molar fraction)

0.33

6.5

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.2 (molar fraction)

0.33

5.1

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.15 (molar fraction)

0.33

9.1

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.1 (molar fraction)

0.33

4.4

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.05 (molar fraction)

0.33

1.67

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.2 (molar fraction)

0.33

0.61

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.15 (molar fraction)

0.33

0.41

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

Km (mM) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.1 (molar fraction)

0.33

0.26

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.05 (molar fraction)

0.12

0.16

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Acetonitrile

0.2 (molar fraction)

0.12

0.65

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Acetonitrile

0.15 (molar fraction)

0.12

1.17

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Acetonitrile

0.1 (molar fraction)

0.12

1.72

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Acetonitrile

0.05 (molar fraction)

0.12

1.12

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.2 (molar fraction)

0.12

0.35

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.15 (molar fraction)

0.12

0.68

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.1 (molar fraction)

0.12

0.54

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethylformamide (DMF)

0.05 (molar fraction)

0.12

0.38

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.2 (molar fraction)

0.12

0.28

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.15 (molar fraction)

0.12

0.28

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

Activity - Michaelis-Menten

kcat (/s) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

0.1 (molar fraction)

0.12

0.18

1/s

100 mM potassium phosphate

60°C

1-Pentanol

Pentanal

0.1 mM NAD+

—

Classical aqueous control (in 1/s)

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Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Alcohol dehydrogenase from Aeropyrum pernix

Enzyme Description

Sequence

Experimental Data (24 measurements)

Visualization : Activity — Michaelis-Menten

Structure

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