Inorganic pyrophosphatase from Saccharomyces cerevisiae

Enzyme Description

Extremophile
No
EC Number

Sequence

Length: 287 amino acids
MTYTTRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKENNIFNMVVEIPRWTNAKLEITKEETLNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNVSHPETKAVGDNDPIDVLEIGETIAYTGQVKQVKALGIMALLDEGETDWKVIAIDINDPLAPKLNDIEDVEKYFPGLLRATNEWFRIYKIPDGKPENQFAFSGEAKNKKYALDIIKETHDSWKQLIAGKSSDSKGIDLTNVTLPDTPTYSKAASDAIPPASPKADAPIDKSIDKWFFISGSV
Rodrigo Grazinoli-Garrido et al. (2004) β€” Inactivation of yeast inorganic pyrophosphatase by organic solvents
Anais da Academia Brasileira de CieΜ‚ncias  Β· doi:10.1590/s0001-37652004000400006 β†—  Β· Activity - Classical
50 measurements
Database ID
UniProt: P00817 β†—
Sequence Annotation
Inferred
Protein Source
Commercially purchased

Experimental Data (50 measurements)

50 measurements β€” page 3 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 0.33M 1700 1736 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 0.85M 1700 1583 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 1.7M 1700 1181 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 2.5M 1700 889 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent Ethanol 3.4M 1700 736 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 37Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent 1-Propanol 0.25M 172 164 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent 1-Propanol 0.65M 172 148 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent 1-Propanol 1.3M 172 113 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent 1-Propanol 1.95M 172 71 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent 1-Propanol 2.6M 172 49 pyrophosphate concentration/(mg*min) 100 mM HEPES-Tris, 5mM MgClβ‚‚, 150 mM KCl 7.5 5Β°C 2 mM Tetrasodium pyrophosphate inorganic phosphate β€” β€” Classical aqueous control (in pyrophosphate concentration/(mg*min))
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Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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