Inorganic pyrophosphatase from Saccharomyces cerevisiae

Enzyme Description

Species

Extremophile

EC Number

3.6.1.1 ↗

Sequence

Length: 287 amino acids

MTYTTRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKENNIFNMVVEIPRWTNAKLEITKEETLNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNVSHPETKAVGDNDPIDVLEIGETIAYTGQVKQVKALGIMALLDEGETDWKVIAIDINDPLAPKLNDIEDVEKYFPGLLRATNEWFRIYKIPDGKPENQFAFSGEAKNKKYALDIIKETHDSWKQLIAGKSSDSKGIDLTNVTLPDTPTYSKAASDAIPPASPKADAPIDKSIDKWFFISGSV

Rodrigo Grazinoli-Garrido et al. (2004) — Inactivation of yeast inorganic pyrophosphatase by organic solvents

Anais da Academia Brasileira de Ciências · doi:10.1590/s0001-37652004000400006 ↗ · Activity - Classical

50 measurements ▼

Database ID

UniProt: P00817 ↗

Sequence Annotation

Inferred

Protein Source

Commercially purchased

Experimental Data (50 measurements)

50 measurements — page 2 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Acetone	0.25M	1700	1762	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Acetone	0.7M	1700	1617	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Acetone	1.4M	1700	1498	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Acetone	2.1M	1700	1242	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Acetone	2.8M	1700	970	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Ethanol	2.5M	172	75	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	1.25M	172	159	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	2.5M	172	132	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	3.75M	172	98	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	5M	172	71	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	0.5M	1700	1603	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	1.25M	1700	1507	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	2.5M	1700	1397	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	3.75M	1700	1137	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	5M	1700	945	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	37°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Ethanol	0.33M	172	164	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Ethanol	0.85M	172	141	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Ethanol	1.7M	172	109	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Methanol	0.5M	172	162	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))
Activity - Classical	Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent	Ethanol	3.4M	172	45	pyrophosphate concentration/(mg*min)	100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl	7.5	5°C	2 mM Tetrasodium pyrophosphate	inorganic phosphate	—	—	Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Activity (in pyrophosphate concentration/(mg*min)) measured by absorbance spectrophotometry (colorimetric method, inorganic phosphate, molybdate and phosphomolybdic acid reaction product absorbance measurement, Lowry-Lopez method) in the presence of organic solvent

1-Propanol

1.95M

1700

1285

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

0.65M

172

153

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

1.3M

172

132

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

1.95M

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

2.6M

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

0.25M

172

169

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

0.7M

172

162

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

1.4M

172

143

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

2.1M

172

109

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

2.8M

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

0.25M

1700

1898

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

0.65M

1700

1685

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

1.3M

1700

1600

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

0.25M

172

158

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

2.6M

1700

842

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

0.25M

1700

1719

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

0.65M

1700

1651

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

1.3M

1700

1472

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

1.95M

1700

1157

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Isopropanol

2.6M

1700

996

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

0.25M

1700

1762

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

0.7M

1700

1617

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

1.4M

1700

1498

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

2.1M

1700

1242

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Acetone

2.8M

1700

970

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

2.5M

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

1.25M

172

159

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

2.5M

172

132

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

3.75M

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

0.5M

1700

1603

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

1.25M

1700

1507

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

2.5M

1700

1397

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

3.75M

1700

1137

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

1700

945

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

0.33M

172

164

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

0.85M

172

141

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

1.7M

172

109

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Methanol

0.5M

172

162

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

3.4M

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

0.33M

1700

1736

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

0.85M

1700

1583

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

1.7M

1700

1181

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

2.5M

1700

889

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

Ethanol

3.4M

1700

736

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

37°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

0.25M

172

164

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

0.65M

172

148

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

1.3M

172

113

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

1.95M

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

Activity - Classical

1-Propanol

2.6M

172

pyrophosphate concentration/(mg*min)

100 mM HEPES-Tris, 5mM MgCl₂, 150 mM KCl

7.5

5°C

2 mM Tetrasodium pyrophosphate

inorganic phosphate

—

Classical aqueous control (in pyrophosphate concentration/(mg*min))

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Inorganic pyrophosphatase from Saccharomyces cerevisiae

Enzyme Description

Sequence

Experimental Data (50 measurements)

Visualization : Activity — Classical

Structure

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