Levansucrase from Bacillus subtilis

Enzyme Description

Species

Extremophile

EC Number

2.4.1.10 ↗

Sequence

Length: 473 amino acids

MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK

E. Castillo et al. (2004) — Synthesis of levan in water-miscible organic solvents

Journal of Biotechnology · doi:10.1016/j.jbiotec.2004.06.003 ↗ · Activity - Classical Stability - Incubation Specificity - Reaction Specificity

38 measurements ▼

Database ID

UniProt: P05655 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, bacterium Bacillus subtilis

Experimental Data (38 measurements)

38 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetone	20%	100.0	86.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	30%	100.0	130.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	30%	100.0	86.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetone	30%	100.0	84.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	40%	100.0	83.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	20%	100.0	97.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	83%	44.9	83.2	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	75%	44.9	82.4	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	63%	44.9	83.5	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	50%	44.9	83.2	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	30%	44.9	74.1	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	15%	44.9	54.6	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	83%	100.0	75.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	75%	100.0	54.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	63%	100.0	51.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	50%	100.0	63.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	30%	100.0	68.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	15%	100.0	99.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")

Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	5%	100.0	97.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	95%	100.0	0.0	%	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	90%	100.0	26.0	%	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	82.5%	100.0	90.0	%	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	75%	100.0	92.0	%	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	62.5%	100.0	94.0	%	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	50%	100.0	141.0	%	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	30%	100.0	118.0	%	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	15%	100.0	87.0	%	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetone	50%	100.0	76.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	50%	100.0	79.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	50%	100.0	137.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetone	40%	100.0	79.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	40%	100.0	136.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	5%	100.0	97.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetone	5%	100.0	99.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	10%	100.0	95.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	10%	100.0	98.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetone	10%	100.0	91.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	20%	100.0	121.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetone	20%	100.0	86.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	tert-Butanol	30%	100.0	130.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	30%	100.0	86.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetone	30%	100.0	84.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	40%	100.0	83.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay) in the presence of organic solvent	Acetonitrile	20%	100.0	97.0	%	50 mM phosphate buffer	6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	83%	44.9	83.2	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	75%	44.9	82.4	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	63%	44.9	83.5	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	50%	44.9	83.2	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	30%	44.9	74.1	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Specificity - Reaction Specificity	Transferase (rather than hydrolysis) activity percentage, measured by HPLC after 20 hour incubation at 30°C in the presence of organic solvent	tert-Butanol	15%	44.9	54.6	transferase activity %	50 mM phosphate buffer	6	37°C	100 g/L Sucrose	Reducing sugars	—	—	Classical aqueous control (in transferase activity %)
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	83%	100.0	75.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	75%	100.0	54.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	63%	100.0	51.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	50%	100.0	63.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	30%	100.0	68.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")
Stability - Incubation	Activity after incubation (1 day at 30°C) measured by absorbance spectrophotometry (DNS assay) in aqueous phase	tert-Butanol	15%	100.0	99.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer	Incubation: 6, Assay: 6	30°C	100 g/L Sucrose	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay in aqueous phase ("diluting the sample enough to measure the residual activity in water.")

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Specificity — Reaction Specificity

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Levansucrase from Bacillus subtilis

Enzyme Description

Sequence

Experimental Data (38 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Visualization : Specificity — Reaction Specificity

Structure

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