ω-transaminase from Aspergillus terreus

Enzyme Description

Species

Extremophile

EC Number

2.6.1.42 ↗

Sequence

Length: 333 amino acids

MASMDKVFAGYAARQAILESTETTNPFAKGIAWVEGELVPLAEARIPLLDQGFMHSDLTYDVPSVWDGRFFRLDDHITRLEASCTKLRLRLPLPRDQVKQILVEMVAKSGIRDAFVELIVTRGLKGVRGTRPEDIVNNLYMFVQPYVWVMEPDMQRVGGSAVVARTVRRVPPGAIDPTVKNLQWGDLVRGMFEAADRGATYPFLTDGDAHLTEGSGFNIVLVKDGVLYTPDRGVLQGVTRKSVINAAEAFGIEVRVEFVPVELAYRCDEIFMCTTAGGIMPITTLDGMPVNGGQIGPITKKIWDGYWAMHYDAAYSFEIDYNERNLEHHHHHH

Shuai Qiua et al. (2025) — Computer-aided design for enhancing the tolerance of ω-amine transaminase against methanol as a cosolvent

Bioorganic Chemistry · doi:10.1016/j.bioorg.2025.108913 ↗ · Activity - Classical Activity - Michaelis-Menten Stability - Half-life

128 measurements ▼

Database ID

PDB: 4CE5 ↗

Sequence Annotation

Explicit - Provided PDB Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (128 measurements)

128 measurements — page 3 of 7

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
N325S	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	6.35	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23I/T200G	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	11.52	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23I/Q294K	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	13.2	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23I/E117V	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	13.52	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T200G/Q294K	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	11.2	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
E117V/T200G	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	14.56	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
E117V/Q294K	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	15.2	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23I/T200G/Q294K	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	17.52	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23I/E117V/T200G	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	18.56	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23I/E117V/Q294K	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	22.16	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
E117V/T200G/Q294K	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	20.56	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23I/E117V/T200G/Q294K	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	28.32	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23I	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	9.6	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23R	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	2.8	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23E	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	8.6	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23S	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	9.3	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23G	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	3.8	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23A	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	9.1	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23N	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	7.4	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)
T23C	Stability - Half-life	Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent	Methanol	30%	—	6.23	6.5	minutes	Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene	(R)-1-(1-naphthyl)ethylamine , Acetophenone	Assay: 0.1 mM PLP	—	Wild-type control with organic solvent (in minutes)

T23I/T200G

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

1.29

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

T23I/Q294K

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

1.69

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

T23I/E117V

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

2.7

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

T200G/Q294K

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

2.58

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

E117V/T200G

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

3.3

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

E117V/Q294K

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

3.91

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

T23I/T200G/Q294K

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

3.14

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

T23I/E117V/T200G

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

5.28

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

T23I/E117V/Q294K

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

7.57

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

E117V/T200G/Q294K

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

9.58

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

T23I/E117V/T200G/Q294K

Activity - Classical

Activity measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.56

11.88

U/mg

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in U/mg)

T23I/E117V/T200G/Q294K

Activity - Michaelis-Menten

kcat (1/min) measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.022

0.449

minutes

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/E117V/T200G/Q294K

Activity - Michaelis-Menten

Km (mM) measured by HPLC in the presence of organic solvent

Methanol

30%

—

5.4

1.6

minutes

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/E117V/T200G/Q294K

Activity - Michaelis-Menten

kcat/Km (1/(min*mM)) measured by HPLC in the presence of organic solvent

Methanol

30%

—

0.0041

0.28

minutes

50 mM PBS buffer

30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

A2S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

4.75

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

K6S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

4.32

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

I17S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

4.15

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.31

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

L41S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.31

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

L48S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

1.11

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

L49S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.58

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

R88S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

2.15

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

K99S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.71

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

F115S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.22

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

10.14

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

I119S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.24

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

L124S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.48

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

R128S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

2.31

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

G129S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.11

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

R131S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.65

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E133S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.05

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

V136S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.62

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

V147S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.92

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.37

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

D224S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.53

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

R232S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

3.21

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.48

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

K300S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.31

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

N322S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.07

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E323S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.17

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

N325S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.35

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/T200G

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

11.52

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/Q294K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

13.2

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/E117V

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

13.52

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200G/Q294K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

11.2

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117V/T200G

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

14.56

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117V/Q294K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

15.2

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/T200G/Q294K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

17.52

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/E117V/T200G

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

18.56

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/E117V/Q294K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

22.16

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117V/T200G/Q294K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

20.56

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I/E117V/T200G/Q294K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

28.32

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23I

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23R

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

2.8

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23E

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.3

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23G

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

3.8

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23A

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.1

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23N

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23C

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.5

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23Q

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.7

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23H

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23L

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.9

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23M

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.5

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23P

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23Y

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.9

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23D

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23F

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

4.7

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23W

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.1

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T23V

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.9

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117T

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

10.2

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117D

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

10.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117F

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

1.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117H

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.2

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117V

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

10.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117Q

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117I

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117R

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117G

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.7

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117A

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.1

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117N

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.9

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117C

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.9

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117L

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

3.9

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117M

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117P

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.8

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117Y

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

4.7

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

E117W

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200Q

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

4.5

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200N

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200G

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

10.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.8

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200A

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.7

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200D

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200F

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.2

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200H

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200V

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200I

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.3

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200R

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.2

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200C

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200L

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.9

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200M

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200P

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200Y

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.5

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200W

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.7

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

T200K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.3

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294S

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

9.5

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294N

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

3.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294G

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

8.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294C

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.7

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294T

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

3.8

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294A

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294H

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.8

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294I

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

1.9

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294L

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

3.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294K

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

10.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294Y

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

3.1

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294R

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

4.6

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294E

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.0

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294M

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

4.8

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294P

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.4

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294D

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.1

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294F

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

6.8

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294W

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

7.2

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

Q294V

Stability - Half-life

Half-life (in minutes), measured after incubation at 30°C, with HPLC assay in the presence of organic solvent

Methanol

30%

—

6.23

5.5

minutes

Incubation: 50 mM PBS buffer, Assay: 50 mM PBS buffer

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

20 mM (R)-1-Phenylethylamine, 20 mM 1-Acetylnaphthalene

(R)-1-(1-naphthyl)ethylamine, Acetophenone

Assay: 0.1 mM PLP

—

Wild-type control with organic solvent (in minutes)

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Mutations in this dataset (109)

▼

A2S K6S I17S T23A T23C T23D T23E T23F T23G T23H T23I T23I/E117V T23I/E117V/Q294K T23I/E117V/T200G T23I/E117V/T200G/Q294K T23I/Q294K T23I/T200G T23I/T200G/Q294K T23K T23L T23M T23N T23P T23Q T23R T23S T23V T23W T23Y L41S L48S L49S R88S K99S F115S E117A E117C E117D E117F E117G E117H E117I E117K E117L E117M E117N E117P E117Q E117R E117S E117T E117V E117V/Q294K E117V/T200G E117V/T200G/Q294K E117W E117Y I119S L124S R128S G129S R131S E133S V136S V147S T200A T200C T200D T200F T200G T200G/Q294K T200H T200I T200K T200L T200M T200N T200P T200Q T200R T200S T200V T200W T200Y D224S R232S Q294A Q294C Q294D Q294E Q294F Q294G Q294H Q294I Q294K Q294L Q294M Q294N Q294P Q294R Q294S Q294T Q294V Q294W Q294Y K300S N322S E323S N325S

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Visualization : Activity — Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Visualization : Stability — Half-life

Mutation Effect

Select a condition below. Conditions with >50 mutations show a heatmap, ranked lollipop, or ranked lollipop; others show paired bars per mutation.

Structure

🧬

No structure available for this protein.

ω-transaminase from Aspergillus terreus

Enzyme Description

Sequence

Experimental Data (128 measurements)

Visualization : Activity — Classical

Mutation Effect

Visualization : Activity — Michaelis-Menten

Visualization : Stability — Half-life

Mutation Effect

Structure

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