5'-nucleotidase from Shewanella violacea

Enzyme Description

Species

Shewanella violacea ↗

Extremophile

Yes "psychrophilic" and "piezophilic" organism

EC Number

3.1.3.5 ↗

Sequence

Length: 569 amino acids

MKNKLIKGLVATAVLAALAGCGSDNDDKDVVLPATCAEAGEACTIFTVLHTNDNHGRFFENKYGEYGMAARKTLIDQIRAEVEANGGETILLSGGDINTGVPESDLQDAVPDFIGMNLIGYDAMAVGNHEFDNPLDVVDMQRKLANFPMLAANIYDKVSGTRYFDPYKVFDVNGLRIAVVGLTTEDTAKIANPEFIGGLEFTDPQEELKKVIQDIKDADAADLIFATTHMGHYADAASGSNAPGDVALARAMTAGDLAAVIGGHSQNPVCMEGDAYDAEFTPGGECKPDVQNGTFIMQAHEWGKYVGQANFEYFNDELHLASYKLIPVNLKAKDADGVRQFIGEEITPDATVLEIMQQYQNAGQEELLEVIGTTDGKLEGERAVVRTQQTNLGRLIAEAQRTKVGADFAVMNSGGVRASIEAGEITYKDVLTVQPFGNSITKSTMTGAEITEYLNAVATVDIGETGSGAYPQLVGIKMDVDCEAKTVAINDINGKGYAVDASYSFTVPSFNAAGGDGYPVLTPVMTGYVDAEVMYTFIKDIGDILLADFAPVASDIQFLNSNSTLACQR

Kiko Fujimori et al. (2019) — Differences in biochemical properties of two 5'-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions

Bioscience, Biotechnology, and Biochemistry · doi:10.1080/09168451.2019.1578641 ↗ · Activity - Classical

24 measurements ▼

Database ID

UniParc: UPI000311FC99 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Purified, bacterium Shewanella violacea

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	28.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	60.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	30%	100.0	9.5	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	20%	100.0	45.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate , inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Ethanol	10%	100.0	22.4	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetone	30%	100.0	18.7	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetone	20%	100.0	19.4	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetone	10%	100.0	35.0	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	3.1	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	3.1	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	10.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Isopropanol	30%	100.0	4.4	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Isopropanol	20%	100.0	4.4	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Isopropanol	10%	100.0	30.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Ethanol	30%	100.0	2.7	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Ethanol	20%	100.0	3.7	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	10%	100.0	84.4	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Methanol	30%	100.0	5.1	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Methanol	20%	100.0	7.8	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Methanol	10%	100.0	37.4	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100.0	11.6	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100.0	15.3	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100.0	40.8	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	13.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	28.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	60.2	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	30%	100.0	9.5	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent	Glycerol	20%	100.0	45.9	%	20 mM Tris-HCl buffer	8	30°C	4 mM ATP (Adenosine triphosphate)	Adenosine 5′-diphosphate, inorganic phosphate	10 mM MgCl₂	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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Structure

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