5'-nucleotidase from Shewanella violacea
Enzyme Description
Species
Extremophile
Yes
"psychrophilic" and "piezophilic" organism
EC Number
Sequence
MKNKLIKGLVATAVLAALAGCGSDNDDKDVVLPATCAEAGEACTIFTVLHTNDNHGRFFENKYGEYGMAARKTLIDQIRAEVEANGGETILLSGGDINTGVPESDLQDAVPDFIGMNLIGYDAMAVGNHEFDNPLDVVDMQRKLANFPMLAANIYDKVSGTRYFDPYKVFDVNGLRIAVVGLTTEDTAKIANPEFIGGLEFTDPQEELKKVIQDIKDADAADLIFATTHMGHYADAASGSNAPGDVALARAMTAGDLAAVIGGHSQNPVCMEGDAYDAEFTPGGECKPDVQNGTFIMQAHEWGKYVGQANFEYFNDELHLASYKLIPVNLKAKDADGVRQFIGEEITPDATVLEIMQQYQNAGQEELLEVIGTTDGKLEGERAVVRTQQTNLGRLIAEAQRTKVGADFAVMNSGGVRASIEAGEITYKDVLTVQPFGNSITKSTMTGAEITEYLNAVATVDIGETGSGAYPQLVGIKMDVDCEAKTVAINDINGKGYAVDASYSFTVPSFNAAGGDGYPVLTPVMTGYVDAEVMYTFIKDIGDILLADFAPVASDIQFLNSNSTLACQR
Kiko Fujimori et al. (2019)
β Differences in biochemical properties of two 5'-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions
Bioscience, Biotechnology, and Biochemistry
Β· doi:10.1080/09168451.2019.1578641 β
Β· Activity - Classical
▼
Database ID
UniParc: UPI000311FC99 β
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Shewanella violacea
Experimental Data (24 measurements)
24 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 22.4 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetone | 30% | 100.0 | 18.7 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetone | 20% | 100.0 | 19.4 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetone | 10% | 100.0 | 35.0 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetonitrile | 30% | 100.0 | 3.1 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetonitrile | 20% | 100.0 | 3.1 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Acetonitrile | 10% | 100.0 | 10.2 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Isopropanol | 30% | 100.0 | 4.4 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Isopropanol | 20% | 100.0 | 4.4 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Isopropanol | 10% | 100.0 | 30.9 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 2.7 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 3.7 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Glycerol | 10% | 100.0 | 84.4 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 5.1 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 7.8 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 37.4 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100.0 | 11.6 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 15.3 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 40.8 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by colorimetric assay (Fiske-Subbarow method, absorbance measurement of the reduced reaction product of inorganic phosphate with ammonium molybdate, 740 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 13.9 | % | 20 mM Tris-HCl buffer | 8 | 30Β°C | 4 mM ATP (Adenosine triphosphate) | Adenosine 5β²-diphosphate , inorganic phosphate | 10 mM MgClβ | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.