D-2-hydroxyacid dehydrogenase (gene D2HDH) from Haloferax mediterranei

Enzyme Description

Species

Haloferax mediterranei ↗

Extremophile

Yes "halophilic" organism

EC Number

1.1.1.28 ↗

Sequence

Length: 308 amino acids

MHIERLAVDESVGRAMPPQRFIEALSDLGVPVEFAGEDEQFGPGDAVASFGHRDAFLDADWVHCIRAGYDEFPVGVYEEAGTYLTNSTGIHGTTVGETVAGYMLTFARRLHAYRDAQHDHAWDLPRYEEPFTLAGERVCVVGLGTLGRGVVDRAAALGMEVVGVRRSGDPVDNVSTVYTPDRLHEAIADARFVVLATPLTDETEGMVAAPEFETMREDASLVNVARGPVVVESDLVAALDSGDIAGAALDVFSEEPLPEDSPLWDFEDVLITPHVSAATSKYHEDVAALIRENIEKIATGDELTNRVV

Jessica Domenech et al. (2025) — Potassium binding by carbonyl clusters, halophilic adaptation and catalysis of Haloferax mediterranei D-2-hydroxyacid dehydrogenase

Communications Biology · doi:10.1038/s42003-025-08587-7 ↗ · Activity - Classical

24 measurements ▼

Database ID

PDB: 9IBE ↗

Sequence Annotation

Explicit - Provided PDB Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli

Experimental Data (24 measurements)

24 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	2.21	1.93	U/mg	50 mM Tris-HCl buffer, 2M NaCl	8	40°C	6.5 mM 2-Oxohexanoic acid	D-2-hydroxyhexanoic acid	0.3 mM NADH	—	Classical aqueous control (in U/mg)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	2.15	1.86	U/mg	50 mM Tris-HCl buffer, 2M NaCl	8	40°C	5 mM 2-Oxohexanoic acid	D-2-hydroxyhexanoic acid	0.3 mM NADH	—	Classical aqueous control (in U/mg)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	2.08	1.81	U/mg	50 mM Tris-HCl buffer, 2M NaCl	8	40°C	4 mM 2-Oxohexanoic acid	D-2-hydroxyhexanoic acid	0.3 mM NADH	—	Classical aqueous control (in U/mg)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	1.83	1.57	U/mg	50 mM Tris-HCl buffer, 2M NaCl	8	40°C	2 mM 2-Oxohexanoic acid	D-2-hydroxyhexanoic acid	0.3 mM NADH	—	Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Glycerol

20%

2.21

1.39

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

6.5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

2.31

0.7

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

12.5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

2.28

0.69

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

10 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

2.25

0.68

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

8 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

2.21

0.67

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

6.5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

2.15

0.65

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

2.08

0.63

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

4 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

1.83

0.55

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

2 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

1.46

0.44

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

1 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Glycerol

20%

2.31

1.48

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

12.5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Glycerol

20%

2.28

1.46

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

10 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Glycerol

20%

2.25

1.43

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

8 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Ethanol

20%

1.46

1.23

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

1 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Glycerol

20%

2.15

1.33

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Glycerol

20%

2.08

1.27

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

4 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Glycerol

20%

1.83

1.05

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

2 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Glycerol

20%

1.46

0.78

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

1 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Ethanol

20%

2.31

2.02

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

12.5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Ethanol

20%

2.28

1.99

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

10 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Ethanol

20%

2.25

1.96

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

8 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Ethanol

20%

2.21

1.93

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

6.5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Ethanol

20%

2.15

1.86

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

5 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Ethanol

20%

2.08

1.81

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

4 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

Activity - Classical

Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent

Ethanol

20%

1.83

1.57

U/mg

50 mM Tris-HCl buffer, 2M NaCl

40°C

2 mM 2-Oxohexanoic acid

D-2-hydroxyhexanoic acid

0.3 mM NADH

—

Classical aqueous control (in U/mg)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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D-2-hydroxyacid dehydrogenase (gene D2HDH) from Haloferax mediterranei

Enzyme Description

Sequence

Experimental Data (24 measurements)

Visualization : Activity — Classical

Structure

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