Lipase from Kurtzmanomyces sp. I-11

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 432 amino acids

AALPDPNEDPFYSTPSNIETFANGQIIQSRKVPTDIGNSNNAASYQLSYRTTNTQEDAVANVATIWIPAKPSSPPRIFTYQVYEDSTQLDCAPSYSYLTGYDQPNKATAVLDTPIVISWALQQGYYVVSADHEGARSAFIAGYEEGMAALDGIRALRNYAKLPQDSAVGAYGYSGGAHATVWATSLAAAYAPEINFIGAAHGGTPVSAKDTFTFINGGFFAGFAIAGVSGLANAHPDMEAFIQPRLNAEGVKTLKQIRSRGFCLPEVVTTYPFKNVFALVNDTNLLTEQPISGILQQETLVQSEASYAVPVPKFPRFLWHAALDEIVPYVPVTEYVKEQCAKGANINFNTYPIAEHLTAEIFGLVPGLWFLSQAYEGKAPAVQCGTALPAAPSAQQVLGNDLANQLSSLNGKQSPFGKPFGPISPTSLDKLL

Koji Kakugawa et al. (2002) — Purification and Characterization of a Lipase from the Glycolipid-Producing Yeast Kurtzmanomyces sp. I-11

Bioscience, Biotechnology, and Biochemistry · doi:10.1271/bbb.66.978 ↗ · Stability - Incubation

56 measurements ▼

Database ID

UniProt: Q8NIP2 ↗

Sequence Annotation

Inferred - from protein name (first 26 residues from UniProt sequence absent from the mature protein)

Protein Source

Purified, fungus Kurtzmanomyces sp. I-11

Experimental Data (56 measurements)

56 measurements — page 2 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethyl Acetate	20%	100	112	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethyl Acetate	40%	100	104	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethyl Acetate	60%	100	114	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethyl Acetate	80%	100	116	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	20%	100	101	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	40%	100	102	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100	86	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	80%	100	100	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	60%	100	111	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	40%	100	98	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	100	66	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	80%	100	10	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	20%	100	107	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	40%	100	93	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100	99	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	80%	100	92	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	20%	100	99	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	40%	100	102	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	60%	100	105	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	80%	100	57	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase

Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	60%	100	141	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Xylene	40%	100	178	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Xylene	60%	100	140	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Xylene	80%	100	122	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	20%	100	98	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	40%	100	118	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	60%	100	111	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexane	80%	100	109	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	20%	100	105	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	40%	100	131	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	60%	100	126	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	80%	100	118	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	20%	100	124	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	40%	100	152	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Xylene	20%	100	135	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	80%	100	64	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100	99	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100	98	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	94	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	80%	100	18	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethyl Acetate	20%	100	112	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethyl Acetate	40%	100	104	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethyl Acetate	60%	100	114	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethyl Acetate	80%	100	116	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	20%	100	101	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	40%	100	102	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	60%	100	86	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	80%	100	100	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	60%	100	111	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	40%	100	98	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	60%	100	66	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	80%	100	10	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	20%	100	107	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	40%	100	93	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	60%	100	99	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	80%	100	92	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	20%	100	99	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	40%	100	102	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	60%	100	105	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	80%	100	57	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	20%	100	113	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	40%	100	110	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	20%	100	104	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	80%	100	98	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isobutanol	20%	100	131	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isobutanol	40%	100	135	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isobutanol	60%	100	124	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isobutanol	80%	100	126	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	20%	100	98	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	40%	100	96	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	60%	100	94	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	80%	100	84	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	20%	100	113	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	40%	100	111	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	60%	100	113	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Chloroform	80%	100	25	%	50 mM acetate buffer	5.6	Incubation: 30°C, Assay: 37°C	2.5 mM p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase

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Visualization : Stability — Incubation

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