Lipase (gene LipB) from Pseudomonas fluorescens SBW25

Enzyme Description

Species

Pseudomonas fluorescens SBW25 ↗

Extremophile

No but "thermostable" enzyme

EC Number

3.1.1.3 ↗

Sequence

Length: 469 amino acids

MGIFDYKNLGTEGSKALFADAMAITLYTYHNLDNGFAVGYQHNGLGLGLPATLVGALLGSTDSQGVIPGLPWNPDSEKAALDAVQKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQVEVLGKYDDAGKLLEIGIGFRGTSGPRETLISDSIGDLVSDLLAALGPKDYAKNYAGEAFGGLLKNVADYASAHGLSGKDVLVSGHSLGGLAVNSLADLSVNKWGGFYKDAHYVAYASPTQSAGDKVLNIGYENDPVFRALDGSSFNLSSLGVHDKPHESTTDNIVSFNDHYASTLWNVLPFSIANLPTWLSHLPTGYGDGMTRIVESGFYEQMSRDATVIVANLSDPARANTWVQDLNRNAEPHKGNTFIIGSDGDDLIQGGKGVDFIEGGKGNDTLRDNSGHNTFLFSGHFGQDRVIGYQLTDKLVFKDVQGSVDYREHGGDTVISVGGDSVTLVGVSGGLGEVVIG

Zhiming Hu et al. (2023) — Characterization of a New Thermostable and Organic Solution-Tolerant Lipase from Pseudomonas fluorescens and Its Application in the Enrichment of Polyunsaturated Fatty Acids

International Journal of Molecular Sciences · doi:10.3390/ijms24108924 ↗ · Stability - Incubation

22 measurements ▼

Database ID

UniProt: C3KBE9 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Bacillus subtilis WB800N

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	10%	100.0	91.25	%	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	Incubation: 150 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	93.12	%	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile	Incubation: 8, Assay: 8	Incubation: 40°C, Assay: 40°C	1 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	Incubation: 150 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Glycerol

30%

100.0

90.65

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

n-Hexane

30%

100.0

92.57

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Cyclohexane

30%

100.0

83.45

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Chloroform

30%

100.0

65.24

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

tert-Butanol

30%

100.0

72.35

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Isopropanol

30%

100.0

76.45

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Acetonitrile

30%

100.0

91.18

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Acetone

30%

100.0

95.74

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Ethanol

30%

100.0

45.79

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Methanol

30%

100.0

56.47

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

30%

100.0

86.53

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Glycerol

10%

100.0

91.87

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

n-Hexane

10%

100.0

102.18

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Cyclohexane

10%

100.0

92.75

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Chloroform

10%

100.0

84.51

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

tert-Butanol

10%

100.0

87.58

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Isopropanol

10%

100.0

95.26

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Acetonitrile

10%

100.0

97.63

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Acetone

10%

100.0

133.18

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Ethanol

10%

100.0

83.04

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Methanol

10%

100.0

91.25

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Stability - Incubation

Activity after incubation (2 hours at 40°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

10%

100.0

93.12

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile

Incubation: 8, Assay: 8

Incubation: 40°C, Assay: 40°C

1 mM p-Nitrophenyl caprylate

Caprylic Acid, p-Nitrophenol

—

Incubation: 150 rpm

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

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Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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Lipase (gene LipB) from Pseudomonas fluorescens SBW25

Enzyme Description

Sequence

Experimental Data (22 measurements)

Visualization : Stability — Incubation

Structure

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