Lipase (gene LipB) from Pseudomonas fluorescens SBW25

Enzyme Description

Species
Pseudomonas fluorescens SBW25 β†—
Extremophile
No but "thermostable" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 469 amino acids
MGIFDYKNLGTEGSKALFADAMAITLYTYHNLDNGFAVGYQHNGLGLGLPATLVGALLGSTDSQGVIPGLPWNPDSEKAALDAVQKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQVEVLGKYDDAGKLLEIGIGFRGTSGPRETLISDSIGDLVSDLLAALGPKDYAKNYAGEAFGGLLKNVADYASAHGLSGKDVLVSGHSLGGLAVNSLADLSVNKWGGFYKDAHYVAYASPTQSAGDKVLNIGYENDPVFRALDGSSFNLSSLGVHDKPHESTTDNIVSFNDHYASTLWNVLPFSIANLPTWLSHLPTGYGDGMTRIVESGFYEQMSRDATVIVANLSDPARANTWVQDLNRNAEPHKGNTFIIGSDGDDLIQGGKGVDFIEGGKGNDTLRDNSGHNTFLFSGHFGQDRVIGYQLTDKLVFKDVQGSVDYREHGGDTVISVGGDSVTLVGVSGGLGEVVIG
Zhiming Hu et al. (2023) β€” Characterization of a New Thermostable and Organic Solution-Tolerant Lipase from Pseudomonas fluorescens and Its Application in the Enrichment of Polyunsaturated Fatty Acids
International Journal of Molecular Sciences  Β· doi:10.3390/ijms24108924 β†—  Β· Stability - Incubation
22 measurements ▼
Database ID
UniProt: C3KBE9 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Bacillus subtilis WB800N

Experimental Data (22 measurements)

22 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 30% 100.0 90.65 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 30% 100.0 92.57 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Cyclohexane 30% 100.0 83.45 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 30% 100.0 65.24 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase tert-Butanol 30% 100.0 72.35 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 30% 100.0 76.45 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 30% 100.0 91.18 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 30% 100.0 95.74 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 30% 100.0 45.79 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 30% 100.0 56.47 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100.0 86.53 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 10% 100.0 91.87 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase n-Hexane 10% 100.0 102.18 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Cyclohexane 10% 100.0 92.75 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Chloroform 10% 100.0 84.51 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase tert-Butanol 10% 100.0 87.58 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 10% 100.0 95.26 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetonitrile 10% 100.0 97.63 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 10% 100.0 133.18 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 10% 100.0 83.04 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 4% Ethanol, 1% acetonitrile Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 40Β°C 1 mM p-Nitrophenyl caprylate Caprylic Acid , p-Nitrophenol β€” Incubation: 150 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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