(S)-amine-transaminase from Albidovulum sp. SLM16

Enzyme Description

Species

Albidovulum sp. SLM16 ↗

Extremophile

Yes "thermophilic" organism

EC Number

2.6.1.18 ↗

Sequence

Length: 470 amino acids

MGSVMTKITNHLPTAELQALDAAHHMHPFTDDAALARKGARIITRGKGVWLTDSDGHEIIDGMAGLWCVNIGYGRRELAEVAARQMEELCYYNTFFQTSHVPAIALAAKIAQLAPGDLNHVFYAGSGSEANDTNIRLVRRYWDVKGQPQRKVIISRKNAYHGSTMGGASLGGMSGMHAQGGLPIPDIVHINQPYWWGEGGDMTPEEFGIARARELEQKIQEIGADRVAAFIAEPIQGAGGVIVPPETYWPEIQRICDEYGILLIADEVITGFGRTGNWFGSQTMGIRPHIMTIAKGLSSGYAPIGGSIICNEVYRTVAESGEDFNHGYTYSGHPVASAVALRNLEIIEDEGIIDHVRNVAHPYLAEKWKGLTEHPLVGEAQLVGLMGSIALTPDKERRARFAAEPGTVGYRCRERCFANDLIMRHVGDRMIIAPALVIRPEEIDILIERAWKSLDECYAEIKAEGLLKAA

Sebastián L. Márquez et al. (2019) — Characterization of a novel thermostable (S)-amine-transaminase from an Antarctic moderately-thermophilic bacterium Albidovulum sp. SLM16

Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2019.109423 ↗ · Activity - Classical Stability - Incubation

21 measurements ▼

Database ID

UniProt: A0A2S5JKA2 ↗

Sequence Annotation

Inferred - from protein name (99% seq ID with real sequence)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (21 measurements)

21 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 50°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase	Dimethylformamide (DMF)	20%	100.0	25.9	%	Incubation: ?, Assay: 100 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 50°C, Assay: 50°C	1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate	Acetophenone , L-Alanine	10 µM PLP	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Dimethylformamide (DMF)

20%

100.0

6.4

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Ethanol

30%

100.0

0.0

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Ethanol

20%

100.0

4.0

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Ethanol

10%

100.0

24.5

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Methanol

30%

100.0

5.6

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Methanol

20%

100.0

37.4

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Methanol

10%

100.0

53.7

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Acetonitrile

30%

100.0

3.3

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Acetonitrile

20%

100.0

8.8

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Acetonitrile

10%

100.0

23.6

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Dimethylformamide (DMF)

30%

100.0

3.2

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Dimethylformamide (DMF)

10%

100.0

17.8

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100.0

8.8

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100.0

25.2

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Activity - Classical

Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100.0

43.2

100 mM Tris-HCl buffer

50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Classical aqueous control (in %) | 99% seq ID with real sequence

Stability - Incubation

Activity after incubation (30 minutes at 50°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase

Dimethyl Sulfoxide (DMSO)

20%

100.0

96.6

Incubation: ?, Assay: 100 mM Tris-HCl buffer

Incubation: ?, Assay: 8

Incubation: 50°C, Assay: 50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence

Stability - Incubation

Activity after incubation (30 minutes at 50°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase

Acetone

20%

100.0

75.0

Incubation: ?, Assay: 100 mM Tris-HCl buffer

Incubation: ?, Assay: 8

Incubation: 50°C, Assay: 50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence

Stability - Incubation

Activity after incubation (30 minutes at 50°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase

Ethanol

20%

100.0

84.0

Incubation: ?, Assay: 100 mM Tris-HCl buffer

Incubation: ?, Assay: 8

Incubation: 50°C, Assay: 50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence

Stability - Incubation

Activity after incubation (30 minutes at 50°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase

Methanol

20%

100.0

81.5

Incubation: ?, Assay: 100 mM Tris-HCl buffer

Incubation: ?, Assay: 8

Incubation: 50°C, Assay: 50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence

Stability - Incubation

Activity after incubation (30 minutes at 50°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase

Acetonitrile

20%

100.0

47.7

Incubation: ?, Assay: 100 mM Tris-HCl buffer

Incubation: ?, Assay: 8

Incubation: 50°C, Assay: 50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence

Stability - Incubation

Activity after incubation (30 minutes at 50°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase

Dimethylformamide (DMF)

20%

100.0

25.9

Incubation: ?, Assay: 100 mM Tris-HCl buffer

Incubation: ?, Assay: 8

Incubation: 50°C, Assay: 50°C

1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate

Acetophenone, L-Alanine

10 µM PLP

—

Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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(S)-amine-transaminase from Albidovulum sp. SLM16

Enzyme Description

Sequence

Experimental Data (21 measurements)

Visualization : Activity — Classical

Visualization : Stability — Incubation

Structure

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