(S)-amine-transaminase from Albidovulum sp. SLM16
Enzyme Description
Sequence
MGSVMTKITNHLPTAELQALDAAHHMHPFTDDAALARKGARIITRGKGVWLTDSDGHEIIDGMAGLWCVNIGYGRRELAEVAARQMEELCYYNTFFQTSHVPAIALAAKIAQLAPGDLNHVFYAGSGSEANDTNIRLVRRYWDVKGQPQRKVIISRKNAYHGSTMGGASLGGMSGMHAQGGLPIPDIVHINQPYWWGEGGDMTPEEFGIARARELEQKIQEIGADRVAAFIAEPIQGAGGVIVPPETYWPEIQRICDEYGILLIADEVITGFGRTGNWFGSQTMGIRPHIMTIAKGLSSGYAPIGGSIICNEVYRTVAESGEDFNHGYTYSGHPVASAVALRNLEIIEDEGIIDHVRNVAHPYLAEKWKGLTEHPLVGEAQLVGLMGSIALTPDKERRARFAAEPGTVGYRCRERCFANDLIMRHVGDRMIIAPALVIRPEEIDILIERAWKSLDECYAEIKAEGLLKAA
SebastiΓ‘n L. MΓ‘rquez et al. (2019)
β Characterization of a novel thermostable (S)-amine-transaminase from an Antarctic moderately-thermophilic bacterium Albidovulum sp. SLM16
Enzyme and Microbial Technology
Β· doi:10.1016/j.enzmictec.2019.109423 β
Β· Activity - Classical Stability - Incubation
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Database ID
UniProt: A0A2S5JKA2 β
Sequence Annotation
Inferred - from protein name (99% seq ID with real sequence)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (21 measurements)
21 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 6.4 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 0.0 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 4.0 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 24.5 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 5.6 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 37.4 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 53.7 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Acetonitrile | 30% | 100.0 | 3.3 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Acetonitrile | 20% | 100.0 | 8.8 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Acetonitrile | 10% | 100.0 | 23.6 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100.0 | 3.2 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 17.8 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 8.8 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 25.2 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Activity - Classical | Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 43.2 | % | 100 mM Tris-HCl buffer | 8 | 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Classical aqueous control (in %) | 99% seq ID with real sequence |
| Stability - Incubation | Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 96.6 | % | Incubation: ?, Assay: 100 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence |
| Stability - Incubation | Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase | Acetone | 20% | 100.0 | 75.0 | % | Incubation: ?, Assay: 100 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence |
| Stability - Incubation | Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase | Ethanol | 20% | 100.0 | 84.0 | % | Incubation: ?, Assay: 100 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence |
| Stability - Incubation | Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase | Methanol | 20% | 100.0 | 81.5 | % | Incubation: ?, Assay: 100 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence |
| Stability - Incubation | Activity after incubation (30 minutes at 50Β°C), measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in aqueous phase | Acetonitrile | 20% | 100.0 | 47.7 | % | Incubation: ?, Assay: 100 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 50Β°C, Assay: 50Β°C | 1 mM (S)-1-Phenylethylamine, 1 mM Pyruvate | Acetophenone , L-Alanine | 10 Β΅M PLP | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, 99% seq ID with real sequence |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.