Lipase from Psychrobacter celer PU3

Enzyme Description

Extremophile
Yes "psyrchor-tolerant" organism, "psychrophilic" enzyme
EC Number

Sequence

Length: 355 amino acids
MTLSLYIRAALTPRTFLPILGSISTGVLLMTMQTISAQAAGSFYNCANATGCQKVDSKWWTSSHTDTKYPLVMTHGLVGFTKLLGIDYFYGIPQTLMKGGSEVYATKTSSFNDSRVRGEQLLRQVKTISAVSGSPKVNLLGHSHGGHDIRYVAGVAPQYVASVTAVASPGQGAALADWVVEQIDNDRINGEYNTATKAALTLFGFLGDFMDLNAGIPIDELQDQDGFGAVTALTTDYTKDFNREFSAAMPTSYCGQPTAIKVNGISYYSFSGVDQVTNVLDPTDYMMLATSQAYDDPNDGLVSACSSRLGYVIRDDYKMNHLDSVNQLLGLVAWGQPNPKTVYRTQVNRLKNANL
Palanichamy Esakkiraj et al. (2022) β€” Functional and molecular characterization of a cold-active lipase from Psychrobacter celer PU3 with potential antibiofilm property
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2022.04.174 β†—  Β· Activity + Stability - Incubation
21 measurements
Database ID
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (21 measurements)

21 measurements β€” page 2 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (1 hour at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 10% 39 28 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
1 2
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Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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