Lipase from Psychrobacter celer PU3

Enzyme Description

Species
Psychrobacter celer PU3 β†—
Extremophile
Yes "psyrchor-tolerant" organism, "psychrophilic" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 355 amino acids
MTLSLYIRAALTPRTFLPILGSISTGVLLMTMQTISAQAAGSFYNCANATGCQKVDSKWWTSSHTDTKYPLVMTHGLVGFTKLLGIDYFYGIPQTLMKGGSEVYATKTSSFNDSRVRGEQLLRQVKTISAVSGSPKVNLLGHSHGGHDIRYVAGVAPQYVASVTAVASPGQGAALADWVVEQIDNDRINGEYNTATKAALTLFGFLGDFMDLNAGIPIDELQDQDGFGAVTALTTDYTKDFNREFSAAMPTSYCGQPTAIKVNGISYYSFSGVDQVTNVLDPTDYMMLATSQAYDDPNDGLVSACSSRLGYVIRDDYKMNHLDSVNQLLGLVAWGQPNPKTVYRTQVNRLKNANL
Palanichamy Esakkiraj et al. (2022) β€” Functional and molecular characterization of a cold-active lipase from Psychrobacter celer PU3 with potential antibiofilm property
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2022.04.174 β†—  Β· Activity + Stability - Incubation
21 measurements ▼
Database ID
UniParc: UPI002184A1B8 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (21 measurements)

21 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (12 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 10% 18 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (24 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Xylene 10% 25 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (24 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Chloroform 10% 25 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (24 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 25 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (24 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 10% 25 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (24 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Acetonitrile 10% 25 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (24 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 10% 25 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (24 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 10% 25 54 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (12 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Xylene 10% 18 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (12 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Chloroform 10% 18 12 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (12 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 18 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (1 hour at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 10% 39 38 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (12 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Acetonitrile 10% 18 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (12 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 10% 18 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (12 hours at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 10% 18 39 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (1 hour at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Xylene 10% 39 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (1 hour at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Chloroform 10% 39 12 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (1 hour at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 39 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (1 hour at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 10% 39 0 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
Activity + Stability - Incubation Activity after incubation (1 hour at ? temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Acetonitrile 10% 39 8 U ? ? ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Water-incubated control (in U), assay in the presence of organic solvent | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase
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Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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