Laccase (gene lcc1) from Kabatiella bupleuri G3

Enzyme Description

Species

Kabatiella bupleuri G3 ↗

Extremophile

Yes "psychrophilic" organism

EC Number

1.10.3.2 ↗

Sequence

Length: 645 amino acids

MHLQKLSGLCLLAFASVSWADAVPGDRYLWGADRMGKMVRRATTTSKASSTTAKASTSSVKTSSVSSASSKSSSNIVASVSSSPPASSASSASSTSSLVADAACTHGPNSRACWGNGYSIKTDFDLKHPTTGNTVTYNLEITNTTCNPDGNGDMPCYLINNQFPGPTIVADWGDKVIINVKNSLQNNGTSLHWHGVRQLSSVGSDGVGGITECPIAPGQSRTYSFLATQFGTSWYHSHYSSQYGMGVVGQVVINGPSSSNYDIDLGTFPITDLYYGLANNVNEMTLHNLQNNLPPPDGSNILVNGKGKSAKGGSYSQMSLTKGKKHRLRLINTSVDNAVRVKLDGHSFTVMSADFIPIKPLTNQGWILLAVGQRYDVVFTANQTPGNYFFRAEVATDCFSGNTGKGRALFTYSEVTAAEPTDSNEVAPTNGCTELNTVPYWTQAVDQSQYAAQVKTLKTGVAPGLTTNGQNFALWNLDTRAMMVRWDRPTIQYVFEGNTSYPDPYAVIEIPTEGTWTYWLIQMGPQNPPLPHPIHLHGHDFFVLGSGAGTFDATTAVLNFANPPRRDTSILPGLGWLLISFPANNPGIWLMHCHIAWHISEGLGVQFIEAKNSIAMPDKTTFNAECDSWRTYSKNMAWPQIDSGL

Katarzyna M. Wi≈õniewska et al. (2021) — Novel Cold-Adapted Recombinant Laccase KbLcc1 from Kabatiella bupleuri G3 IBMiP as a Green Catalyst in Biotransformation

International Journal of Molecular Sciences · doi:10.3390/ijms22179593 ↗ · Activity - Classical Stability - Incubation

48 measurements ▼

Database ID

UniProt: A0A8F5DNG6 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host yeast Pichia pastoris strain PichiaPink^TM 1

Experimental Data (48 measurements)

48 measurements — page 2 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethyl Acetate	40%	100	21	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	1%	100	90	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	60	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	49	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	1%	100	89	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	74	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	20%	100	26	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	40%	100	9	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	1%	100	85	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100	62	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	20%	100	35	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	40%	100	11	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	1%	100	84	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	10%	100	94	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	20%	100	132	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	40%	100	108	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	40%	100	73	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	20%	100	83	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100	83	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	1%	100	90	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	18	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	1%	100	67	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	n-Hexane	40%	100	158	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	n-Hexane	20%	100	140	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	n-Hexane	10%	100	115	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	n-Hexane	1%	100	116	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	40%	100	11	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	20%	100	21	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	66	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	1%	100	93	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	40%	100	6	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	20%	100	21	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	68	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	40%	100	14	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	1%	100	99	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	20%	100	1	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	40%	100	1	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethyl Acetate	1%	100	74	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethyl Acetate	10%	100	34	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethyl Acetate	20%	100	23	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethyl Acetate	40%	100	21	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	1%	100	90	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	60	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	20%	100	49	%	100 mM acetate buffer	3.5	30°C	1 mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	1%	100	89	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100	74	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	20%	100	26	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	40%	100	9	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	1%	100	85	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100	62	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	20%	100	35	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	40%	100	11	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	1%	100	84	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	10%	100	94	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	20%	100	132	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	40%	100	108	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	40%	100	73	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	20%	100	83	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100	83	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	1%	100	90	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethyl Acetate	40%	100	7	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethyl Acetate	20%	100	2	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethyl Acetate	10%	100	35	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethyl Acetate	1%	100	82	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	40%	100	44	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	20%	100	62	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100	84	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	1%	100	91	%	Incubation: 100 mM acetate buffer, Assay: 100 mM acetate buffer	Incubation: 3.5, Assay: 3.5	Incubation: 4°C, Assay: 30°C	1 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Clear about assay

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation