GH39 xylosidase (gene XYL21) from a saline soil sample metagenomic library

Enzyme Description

Species

Na (saline soil sample metagenomic library)

Extremophile

Yes likely halophilic organism (from saline soil) and "halo-tolerant" enzyme

EC Number

3.2.1.37 ↗

Sequence

Length: 501 amino acids

MEKIMISRDNNIPFNKHWKKCIGTGRLGLALQKEYVDHLERLQQDIGFDYIRGHGLFHEDIGIYKEVDVGGTPTPFYNFTYIDRIFDTFLQNNLRPFVELGFMPKKLASGTQTIFYWEGNVTPPAEEKKWEELIYHTVIHFVERYGVEEVLQWPFEVWNEPNLSNFWENADKQAYFQLYKITAKTIKSIHPDLNVGGPAICGGTDEWITDFLHFCHKEQVPVDFISRHAYTSKPPKKKTPDYYYQDLADPNDMLTQLTSVKKLIHDTPYPDLPFHITEYNTSYSPINPIHDTNYNAAYLGKILSHAGDIVSSFSYWTFSDVFEEFDIPRAPFHGGFGLMALHAIRKPTYHLFSFFNKLGDTCLYKDDTMIVTKHRDNTISIVAWNLIHDKGENHDKTITLSIPFSSDDVFLYREITNEHHANPWRVWKQMGRPRFPSKEQITLLKSCDIPFIQTDKLMTENKLLTYQLTMAKNEVTLLHFSDIIDETNTYIGLDDSLLTSY

Zhongyuan Li et al. (2020) — Biochemical characterization of a novel halo/organic-solvents/final-products tolerant GH39 xylosidase from saline soil and its synergic action with xylanase

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.07.079 ↗ · Activity - Classical

42 measurements ▼

Database ID

UniParc: UPI00090C2DE3 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (42 measurements)

42 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	25%	100	11	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	10%	100	88	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	15%	100	76	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	20%	100	68	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	25%	100	55	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	30%	100	45	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	35%	100	38	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	5%	100	191	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	10%	100	48	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	15%	100	17	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	20%	100	14	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	5%	100	112	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	30%	100	6	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	35%	100	3	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	5%	100	176	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	10%	100	153	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	15%	100	156	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	20%	100	147	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	25%	100	122	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	30%	100	110	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	25%	100	11	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	10%	100	88	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	15%	100	76	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	20%	100	68	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	25%	100	55	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	30%	100	45	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	35%	100	38	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	5%	100	191	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	10%	100	48	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	15%	100	17	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	20%	100	14	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Butanol	5%	100	112	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	30%	100	6	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1-Propanol	35%	100	3	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	5%	100	176	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	10%	100	153	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	15%	100	156	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	20%	100	147	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	25%	100	122	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	30%	100	110	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isoamyl Alcohol	35%	100	97	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	25%	100	9	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	10%	100	172	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	15%	100	147	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	20%	100	81	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	25%	100	30	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	30%	100	17	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	35%	100	15	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	5%	100	178	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	10%	100	166	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	15%	100	77	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	20%	100	17	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	5%	100	165	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	30%	100	5	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	35%	100	7	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Ethanol	5%	100	175	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Ethanol	10%	100	168	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Ethanol	15%	100	105	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Ethanol	20%	100	30	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Ethanol	25%	100	15	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Ethanol	30%	100	10	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Ethanol	35%	100	6	%	200 mM citric acid–Na₂HPO₄ buffer	5.5	45°C	2 mM β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %) \| Uncertainty about pH and buffer

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Visualization : Activity — Classical

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