Laccase 6 (gene LACC6) from Pleurotus ostreatus HAUCC 162

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 533 amino acids

MAVAFVALVSLALALVRVEASIGPRGTLNIANKVIQPDGFSRSAVLAGGSYPGPLIKGKTGDRFQINVVNKLADTSMPVDTSIHWHGLFVKGHNWADGPAMVTQCPIVPGHSFLYDFEVPDQAGTFWYHSHLGTQYCDGLRGPLVVYSKNDPHKRLYDVDDESTVLTVGDWYHAPSLSLTGVPHPDSTLFNGLGRSLNGPASPLYVMNVVKGKRYRIRLINTSCDSNYQFSIDGHTFTVIEADGENTQPLQVDQVQIFAGQRYSLVLNANQAVGNYWIRANPNSGDPGFANQMNSAILRYKGARSIDPTTPEQNATNPLREYNLRPLIKKPAPGKPFPGGADHNINLNFAFDPATALFTANNFTFVPPTVPVLLQILSGTRDAHDLAPAGSIYDIKLGDVVEITMPALVFAGPHPLHLHGHTFAVVRSAGSSTYNYENPVRRDVVSIGDDPTDNVTIRFVADNAGPWFLHCHIDWHLDLGFAVVFAEGVNQTAVANPVPEAWNNLCPIYNSSNPSKLLMGTNAIGRLPAPLKA

Rui Zhuo et al. (2019) — The roles of Pleurotus ostreatus HAUCC 162 laccase isoenzymes in decolorization of synthetic dyes and the transformation pathways

Chemosphere · doi:10.1016/j.chemosphere.2019.06.113 ↗ · Activity - Classical Stability - Incubation

80 measurements ▼

Database ID

UniProt: A0A1S6YJE5 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host yeast Pichia pastoris GS115

Experimental Data (80 measurements)

80 measurements — page 3 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	42.39	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	74.69	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	35.9	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	75.36	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	46.98	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	82.87	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	31.39	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	78.24	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	46.05	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	92.54	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	90.51	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	103.52	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	71.04	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	101.41	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	72.51	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	99.41	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	75.76	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	99.17	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	77.98	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	97.43	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Methanol	20%	100.0	66.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Methanol	10%	100.0	91.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Glycerol	20%	100.0	30.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Glycerol	10%	100.0	78.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethylene Glycol	20%	100.0	16.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethylene Glycol	10%	100.0	23.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethylformamide (DMF)	20%	100.0	9.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethylformamide (DMF)	10%	100.0	34.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetonitrile	20%	100.0	19.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetonitrile	10%	100.0	68.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethyl Sulfoxide (DMSO)	20%	100.0	20.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetone	20%	100.0	18.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Acetone	10%	100.0	47.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Isopropanol	20%	100.0	17.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Isopropanol	10%	100.0	58.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	1-Propanol	20%	100.0	7.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	1-Propanol	10%	100.0	42.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethanol	20%	100.0	31.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Ethanol	10%	100.0	74.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm)	Dimethyl Sulfoxide (DMSO)	10%	100.0	56.0	%	50 mM acetic acid-sodium acetic buffer	5	25°C	1 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	32.92	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	78.26	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	41.1	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	58.46	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	68.08	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	96.22	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	71.4	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	87.19	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	71.2	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	87.85	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	69.49	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	86.92	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	77.08	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	53.45	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	90.72	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	76.68	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	34.33	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	66.96	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	34.5	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	84.73	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	42.39	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	74.69	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	35.9	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	75.36	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	46.98	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	82.87	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	31.39	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	78.24	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	46.05	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (24 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	92.54	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100.0	90.51	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	103.52	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	71.04	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	101.41	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	72.51	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	99.41	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	75.76	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	99.17	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	77.98	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	97.43	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	75.28	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100.0	93.66	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	78.72	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	10%	100.0	91.0	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetonitrile	50%	100.0	79.88	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	50%	100.0	56.8	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	74.88	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	10%	100.0	92.6	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethylene Glycol	50%	100.0	80.54	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	10%	100.0	97.54	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (6 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Glycerol	50%	100.0	84.02	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	78.87	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	50%	100.0	62.67	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	85.24	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	50%	100.0	67.64	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	90.2	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	50%	100.0	49.99	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	10%	100.0	87.79	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	50%	100.0	45.99	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (12 hours at 4°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	89.75	%	Incubation: 100mM sodium acetate buffer, Assay: 50 mM acetic acid-sodium acetic buffer	Incubation: 4.8, Assay: 5	Incubation: 4°C, Assay: 25°C	1 mM ABTS	ABTS radical cation	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation