Lipase from Bacillus sp. S1DI 10

Enzyme Description

Species

Extremophile

EC Number

3.4.- ↗

Sequence

Length: 803 amino acids

MKKVSIRAVLSTVAFSVALSSFAMGASANGNSKPALEPSLVKIQGDYNLKSAKKVKVIVELNEESVAEAKKKGVAQSKGKIKKARDEVKKELSKASKTSKVKREYDQVFSGFSAELPANDLEKVASLPGVKAIYPSVEYHTTEVKSKEVSAEEYGSEMDKSIYYVGADQAWKSGYTGKNMTVAVIDTGVDYDHPDLKSAFEKYKGWDFVDDDKDPQETPAGDPKGEATTHGTHVAGTIAADGKIKGVAPDAHLLAYRVLGPGGTGTTEDVIAGIERAVEDGADVMNLSLGDTINNPDLATSIALDWAMEEGVVAVTSNGNSGPANWTVGSPGTSREAISVGATQLPYNLYKTTLTVDNASYASAEVMGFPNDKALLDASGKKYEFVPVGLGKPEDFEGKDVKGKVAVISRGDIAFVDKVDNAKKAGAVATVIYNNVEGTIPDIPGTSLPSIRLSKADGQALVASLAKGSVTGSFTATFDQTVDETMADFSSRGPVVDTWMIKPDISAPGVDIISTVPTNDPSNPHGYGSKQGTSMAAPHVAGAAALILQAHPNYKVEDVKASLMNTTELLRDRNGLVYPHNTQGAGSMRVVDAIKAKTLITPGSHSYGVFYKDKGKQVEKQSFKIKNLSNHSQKYSVKVKFKKSHQAIDVKSTNDLVVNAGKTQKVNLNVKVDAGKLSPGYYEGTITVSNNKEMYDVPTILFVKEPDYPRVTSAYVDVLGNGSFEYGSYLPGGAEKLSYYIYDATLEKGELLGSYTNVEKGFSSATWDGKINGEALPAGTYYLYAEAVKAGQTTGSLGEFEIK

Drishtant Singh et al. (2019) — Characterization of a cold-active, detergent-stable metallopeptidase purified from Bacillus sp. S1DI 10 using Response Surface Methodology

PLOS ONE · doi:10.1371/journal.pone.0216990 ↗ · Activity + Stability - Incubation

50 measurements ▼

Database ID

UniParc: UPI0001D0460C ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (50 measurements)

50 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	40%	100.0	50.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	20%	100.0	63.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	30%	100.0	58.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	40%	100.0	55.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	50%	100.0	50.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	10%	100.0	53.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	20%	100.0	49.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	30%	100.0	41.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	40%	100.0	39.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	50%	100.0	35.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	10%	100.0	58.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	20%	100.0	55.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	30%	100.0	53.6	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	10%	100.0	70.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	50%	100.0	47.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	86.1	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	67.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	66.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100.0	63.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	55.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent

Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	40%	100.0	50.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	20%	100.0	63.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	30%	100.0	58.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	40%	100.0	55.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	50%	100.0	50.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	10%	100.0	53.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	20%	100.0	49.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	30%	100.0	41.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	40%	100.0	39.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	50%	100.0	35.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	10%	100.0	58.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	20%	100.0	55.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	30%	100.0	53.6	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	10%	100.0	70.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	50%	100.0	47.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	86.1	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	67.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	66.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100.0	63.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	55.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	PEG	10%	100.0	79.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	PEG	20%	100.0	67.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	PEG	30%	100.0	57.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	PEG	40%	100.0	44.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	PEG	50%	100.0	38.1	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	40%	100.0	68.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Ethanol	20%	100.0	67.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Ethanol	30%	100.0	59.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Ethanol	40%	100.0	57.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Ethanol	50%	100.0	53.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	10%	100.0	83.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	20%	100.0	72.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	30%	100.0	62.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	40%	100.0	57.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	50%	100.0	53.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	10%	100.0	77.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	20%	100.0	73.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	30%	100.0	70.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Ethanol	10%	100.0	77.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	50%	100.0	66.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetone	10%	100.0	75.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetone	20%	100.0	64.9	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetone	30%	100.0	62.1	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetone	40%	100.0	57.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetone	50%	100.0	54.7	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	10%	100.0	103.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	20%	100.0	109.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	30%	100.0	116.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	40%	100.0	123.0	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent
Activity + Stability - Incubation	Activity after incubation (30 hours at 10°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	50%	100.0	129.8	%	Incubation: ?, Assay: 50 mM phosphate buffer	Incubation: ?, Assay: 7	Incubation: 10°C, Assay: 10°C	1% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in the presence of organic solvent \| Uncertainty about whether assay was really was in the presence of organic solvent

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Visualization : Activity + Stability — Incubation

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