Nitrilase (gene PaCNit) from Pannonibacter carbonis Q4.6

Enzyme Description

Species

Pannonibacter carbonis Q4.6 ↗

Extremophile

No but thermostable enzyme

EC Number

3.5.5.1 ↗

Sequence

Length: 297 amino acids

MAEFPIALCSLNLGFAPALPEAYVAAIDQRMARAKADGARLLMLPEYCIEACLAFRPEALEPTAEMAFLSEVAVAIEPQLAALAARHGLSLLAGSMPWPHEGGGHTNRAIFFTPDGCRHVHDKLSLTPFEKAPEPWVLTPGRKLTVFEVEGVRIAMLICLDVEMPALSCLLAKEHLDLLLVPSMTGKLSGYHRVYDCAKARAVELLTSVALCGCTGVATGTTQNDTNFSGAALYVPCEAELGYTGTAVSLTPTGGEKGEEPYVVVHPPIDAVRRMRAGAAEVWPGAWSAAGIEICRQ

Dejian Liu et al. (2019) — Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6

Biotechnology Letters · doi:10.1007/s10529-019-02661-x ↗ · Activity - Classical

22 measurements ▼

Database ID

UniParc: UPI000D111D56 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	n-Hexane	1%	100	107	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia , Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Isopropanol	1%	100	117	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia , Nicotinic acid	—	—	Correct contol (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Glycerol	5%	100	48	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Dimethyl Sulfoxide (DMSO)	5%	100	48	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Methanol	5%	100	71	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Acetone	5%	100	120	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Diethyl Ether	5%	100	77	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	n-Heptane	5%	100	75	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Ethyl Acetate	5%	100	65	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Chloroform	5%	100	98	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Ethanol	5%	100	93	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	n-Hexane	5%	100	94	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Isopropanol	5%	100	99	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Glycerol	1%	100	132	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Dimethyl Sulfoxide (DMSO)	1%	100	68	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Methanol	1%	100	87	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Acetone	1%	100	89	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Diethyl Ether	1%	100	91	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	n-Heptane	1%	100	92	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Ethyl Acetate	1%	100	94	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Chloroform	1%	100	97	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Ethanol	1%	100	97	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	n-Hexane	1%	100	107	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm)	Isopropanol	1%	100	117	%	50 mM sodium phosphate buffer	7.2	30°C	50 mM 3-Cyanopyridine	Ammonia, Nicotinic acid	—	—	Correct contol (in %)

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Structure

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