Nitrilase (gene PaCNit) from Pannonibacter carbonis Q4.6
Enzyme Description
Species
Extremophile
No
but thermostable enzyme
EC Number
Sequence
MAEFPIALCSLNLGFAPALPEAYVAAIDQRMARAKADGARLLMLPEYCIEACLAFRPEALEPTAEMAFLSEVAVAIEPQLAALAARHGLSLLAGSMPWPHEGGGHTNRAIFFTPDGCRHVHDKLSLTPFEKAPEPWVLTPGRKLTVFEVEGVRIAMLICLDVEMPALSCLLAKEHLDLLLVPSMTGKLSGYHRVYDCAKARAVELLTSVALCGCTGVATGTTQNDTNFSGAALYVPCEAELGYTGTAVSLTPTGGEKGEEPYVVVHPPIDAVRRMRAGAAEVWPGAWSAAGIEICRQ
Dejian Liu et al. (2019)
β Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6
Biotechnology Letters
Β· doi:10.1007/s10529-019-02661-x β
Β· Activity - Classical
▼
Database ID
UniParc: UPI000D111D56 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (22 measurements)
22 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Glycerol | 5% | 100 | 48 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Dimethyl Sulfoxide (DMSO) | 5% | 100 | 48 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Methanol | 5% | 100 | 71 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Acetone | 5% | 100 | 120 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Diethyl Ether | 5% | 100 | 77 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | n-Heptane | 5% | 100 | 75 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Ethyl Acetate | 5% | 100 | 65 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Chloroform | 5% | 100 | 98 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Ethanol | 5% | 100 | 93 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | n-Hexane | 5% | 100 | 94 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Isopropanol | 5% | 100 | 99 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Glycerol | 1% | 100 | 132 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Dimethyl Sulfoxide (DMSO) | 1% | 100 | 68 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Methanol | 1% | 100 | 87 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Acetone | 1% | 100 | 89 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Diethyl Ether | 1% | 100 | 91 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | n-Heptane | 1% | 100 | 92 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Ethyl Acetate | 1% | 100 | 94 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Chloroform | 1% | 100 | 97 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (ammonia absorbance measurement, 640 nm) | Ethanol | 1% | 100 | 97 | % | 50 mM sodium phosphate buffer | 7.2 | 30Β°C | 50 mM 3-Cyanopyridine | Ammonia , Nicotinic acid | β | β | Correct contol (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.