Pectate lyase from Aspergillus luchuensis var. saitoi

Enzyme Description

Species

Extremophile

EC Number

4.2.2.2 ↗

Sequence

Length: 323 amino acids

MTNFKWIVAAAGLLSGQVLAAPTATSTHAKRATVSDAAFGYASMNGGTTGGAGGTTTTVSSYAAFTSAVSGDDAKVVYVDGTIKETADQVKIGSNTSIIGKDANAILEGFGVLVKEKENVIIRNLGVTKVLADNGDAIGIQYSNNVWIDHCDVSSDRDHDKDYYDGLIDITHGSDYVTVSNTFIHDHWKASLVGHSVSNEDEDSGHLTVTYANNYWYNVNSRAPSFRFGTGHIYNSYYLDVSDGINTRDGAQLLVESNQFVDSKKALYSTDDGYAVSNDNDFGDAKSTAEEGTLTSMPYDYTLLGSANVKAAVVGTAGQTLTF

Junya Kamijo et al. (2019) — Identification and characterization of a thermostable pectate lyase from Aspergillus luchuensis var. saitoi

Food Chemistry · doi:10.1016/j.foodchem.2018.10.059 ↗ · Stability - Incubation

64 measurements ▼

Database ID

—

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host yeast Pichia pastoris KM71H

Experimental Data (64 measurements)

64 measurements — page 4 of 4

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
V197D	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)	Ethanol	40%	100	25	22	%	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	0.5% Polygalacturonic acid	Δ4,5-unsaturated oligogalacturonic acids	0.3 mM Ca²⁺	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer
I140V/V197D	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)	Ethanol	40%	100	25	22	%	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	0.5% Polygalacturonic acid	Δ4,5-unsaturated oligogalacturonic acids	0.3 mM Ca²⁺	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer
I140V	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)	Isopropanol	5%	100	108	114	%	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	0.5% Polygalacturonic acid	Δ4,5-unsaturated oligogalacturonic acids	0.3 mM Ca²⁺	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer
V197D	Stability - Incubation	Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)	Isopropanol	5%	100	108	110	%	Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2	Incubation: 8, Assay: 8	Incubation: 30°C, Assay: 30°C	0.5% Polygalacturonic acid	Δ4,5-unsaturated oligogalacturonic acids	0.3 mM Ca²⁺	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

100

—

105

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

10%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

100

—

108

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

20%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

40%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

40%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

20%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

100

—

120

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

10%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

100

—

124

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

10%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

40%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

20%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

20%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

10%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

40%

100

—

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

100

120

110

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

100

120

116

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

100

120

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Methanol

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

100

124

123

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

100

105

106

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

100

105

110

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

100

105

112

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Acetone

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

100

124

117

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

100

108

121

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

100

124

116

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

10%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

10%

100

102

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

10%

100

102

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

20%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V/V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Ethanol

40%

100

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

I140V

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

100

108

114

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

V197D

Stability - Incubation

Activity after incubation (1 hour at 30°C), measured by absorbance spectrophotometry (unsaturated bonds absorbance measurement, 235 nm)

Isopropanol

100

108

110

Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer, 0.3 MM CaCl2

Incubation: 8, Assay: 8

Incubation: 30°C, Assay: 30°C

0.5% Polygalacturonic acid

Δ4,5-unsaturated oligogalacturonic acids

0.3 mM Ca²⁺

—

Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really is in aqueous phase/uncertainty about EC number and reaction buffer

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Mutations in this dataset (4)

WT I140V I140V/V197D V197D

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Pectate lyase from Aspergillus luchuensis var. saitoi

Enzyme Description

Sequence

Experimental Data (64 measurements)

Visualization : Stability — Incubation

Mutation Effect

Structure

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