Aminopeptidase (gene mesoamp) from Mesorhizobium SEMIA300

Enzyme Description

Species

Extremophile

EC Number

3.4.1.1 ↗

Sequence

Length: 418 amino acids

MTTHSRGVSATIDPVKLDRLAEVAIKVGLQLQPGQDLVLTSSIAALPLTRRIVEHAYKAGAGLVTPIFNDDEMTLARYRFGADASFDRAAGWLYEGMAKAFSNNAARLAVRGEDPSLLSAQDPAKVARANKANSMAYQPALEKITGFDINWNIVAYPDLAWAKQVFPGDADDVAVAKLADAIFAASRVDVEDPIGNWKAHNAALRGRTEWLNGHNFHALHFTGPGTDLTVGLADGHEWMGGASTAKNGITCNPNIPTEEVFTTPHARRVEGHVSSTKPLSYQGTLIDEISVRFEEGRIVEAKASKGEDVLKKVLDTDEGARRLGEVALVPHSSPISASGLLFFNTLFDENAACHIALGQCYSKCFVDGASLSQDEIAARGGNKSFIHIDWMIGSDKIDIDGVHKDGSRVPVMRKGEWA

Elwi Machado Sierra et al. (2017) — Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis

Scientific Reports · doi:10.1038/s41598-017-10932-8 ↗ · Stability - Incubation

32 measurements ▼

Database ID

UniParc: UPI00083DE3DB ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Methanol	5%	100	87	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Isopropanol	40%	100	0	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Isopropanol	20%	100	70	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	97	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Isopropanol	5%	100	129	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Ethanol	40%	100	0	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Ethanol	20%	100	111	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	98	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Ethanol	5%	100	104	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Acetone	40%	100	13	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Acetone	20%	100	23	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100	55	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control

Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Cyclohexane	5%	100	97	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	n-Hexane	40%	100	30	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	n-Hexane	20%	100	81	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	n-Hexane	10%	100	117	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	n-Hexane	5%	100	109	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	n-Heptane	40%	100	47	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	n-Heptane	20%	100	55	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	n-Heptane	10%	100	75	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	n-Heptane	5%	100	102	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Chloroform	40%	100	54	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Chloroform	20%	100	41	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Chloroform	10%	100	65	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Chloroform	5%	100	90	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Cyclohexane	40%	100	0	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Cyclohexane	20%	100	0	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Cyclohexane	10%	100	121	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Acetone	5%	100	74	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Methanol	40%	100	40	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Methanol	20%	100	104	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100	80	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Methanol	5%	100	87	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Isopropanol	40%	100	0	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Isopropanol	20%	100	70	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	97	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Isopropanol	5%	100	129	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Ethanol	40%	100	0	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Ethanol	20%	100	111	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	98	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Ethanol	5%	100	104	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Acetone	40%	100	13	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Acetone	20%	100	23	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control
Stability - Incubation	Activity after incubation (45 minutes at 4°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100	55	%	Incubation: ?, Assay: 20 mM Tris-HCl buffer	Incubation: ?, Assay: 8	Incubation: 4°C, Assay: Room temperature	? mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Non-incubated control (in %), assay in aqueous phase \| Clear about assay, uncertainty about control

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Visualization : Stability — Incubation

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