Aminopeptidase (gene mesoamp) from Mesorhizobium SEMIA300

Enzyme Description

Species
Mesorhizobium SEMIA300 β†—
Extremophile
No
EC Number
3.4.1.1 β†—

Sequence

Length: 418 amino acids
MTTHSRGVSATIDPVKLDRLAEVAIKVGLQLQPGQDLVLTSSIAALPLTRRIVEHAYKAGAGLVTPIFNDDEMTLARYRFGADASFDRAAGWLYEGMAKAFSNNAARLAVRGEDPSLLSAQDPAKVARANKANSMAYQPALEKITGFDINWNIVAYPDLAWAKQVFPGDADDVAVAKLADAIFAASRVDVEDPIGNWKAHNAALRGRTEWLNGHNFHALHFTGPGTDLTVGLADGHEWMGGASTAKNGITCNPNIPTEEVFTTPHARRVEGHVSSTKPLSYQGTLIDEISVRFEEGRIVEAKASKGEDVLKKVLDTDEGARRLGEVALVPHSSPISASGLLFFNTLFDENAACHIALGQCYSKCFVDGASLSQDEIAARGGNKSFIHIDWMIGSDKIDIDGVHKDGSRVPVMRKGEWA
Elwi Machado Sierra et al. (2017) β€” Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis
Scientific Reports  Β· doi:10.1038/s41598-017-10932-8 β†—  Β· Stability - Incubation
32 measurements ▼
Database ID
UniParc: UPI00083DE3DB β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (32 measurements)

32 measurements β€” page 1 of 2
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Cyclohexane 5% 100 97 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Hexane 40% 100 30 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Hexane 20% 100 81 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Hexane 10% 100 117 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Hexane 5% 100 109 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Heptane 40% 100 47 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Heptane 20% 100 55 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Heptane 10% 100 75 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase n-Heptane 5% 100 102 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Chloroform 40% 100 54 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Chloroform 20% 100 41 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Chloroform 10% 100 65 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Chloroform 5% 100 90 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Cyclohexane 40% 100 0 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Cyclohexane 20% 100 0 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Cyclohexane 10% 100 121 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Acetone 5% 100 74 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Methanol 40% 100 40 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Methanol 20% 100 104 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
Stability - Incubation Activity after incubation (45 minutes at 4Β°C), measured by absorbance spectrophotometry (p-nitroanilide absorbance measurement, 405 nm) in aqueous phase Methanol 10% 100 80 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: Room temperature ? mM Leucine p-nitroanilide L-Leucine , p-Nitroaniline β€” β€” Non-incubated control (in %), assay in aqueous phase | Clear about assay, uncertainty about control
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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