Esterase (gene EstDZ3) from Dictyoglomus thermophilum Ch5.6.S

Enzyme Description

Species

Dictyoglomus thermophilum Ch5.6.S ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

3.1.1.1 ↗

Sequence

Length: 256 amino acids

MTENREPVVLKNQGQKIFGVIHIPEKTPAPFVLFCHGFTGTKIEPHRIFVKTAEALAKEGIGALRIDFRGSGDSEGSFKDMTVEGEVSDAMVAIEYLSQNNLVDKEKIGILGLSMGGAVASITSGRNPLIKSCVLWSAVCHFDIFFNRSPEEVSRIKDYGDFIDLGGNPVGKKFLSEIVNIKPLEEIKKRSIPVLIIHGSGDMVVPIQHAYDYFNGLKDTHKVKLEIIEGADHTFNSIEWEEKVIEKTVNWFKETL

Dimitra Zarafeta et al. (2016) — EstDZ3: A New Esterolytic Enzyme Exhibiting Remarkable Thermostability

Frontiers in Microbiology · doi:10.3389/fmicb.2016.01779 ↗ · Activity - Classical Stability - Incubation

23 measurements ▼

Database ID

UniProt: A0A1S5RMF8 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (23 measurements)

23 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	50%	100.0	74.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	50%	100.0	71.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	50%	100.0	81.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	30%	100.0	23.8	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	30%	100.0	25.8	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	10%	100.0	51.2	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isooctane	30%	100.0	33.6	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isooctane	10%	100.0	53.6	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	29.5	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	109.6	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	30%	100.0	8.3	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	10%	100.0	50.4	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100.0	2.8	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	30%	100.0	28.1	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100.0	109.6	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	114.1	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	30%	100.0	26.5	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100.0	93.0	%	25 mM Tris-HCl, 0.05% Triton X-100	8	75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	50%	100.0	96.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	50%	100.0	69.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isooctane	50%	100.0	74.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	50%	100.0	59.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	50%	100.0	72.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	50%	100.0	74.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	50%	100.0	71.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	50%	100.0	81.0	%	Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 75°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	Yes, "vigorously agitated during incubation"	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation