Esterase (gene EstDZ3) from Dictyoglomus thermophilum Ch5.6.S
Enzyme Description
Extremophile
Yes
"hyperthermophilic" organism
EC Number
Sequence
MTENREPVVLKNQGQKIFGVIHIPEKTPAPFVLFCHGFTGTKIEPHRIFVKTAEALAKEGIGALRIDFRGSGDSEGSFKDMTVEGEVSDAMVAIEYLSQNNLVDKEKIGILGLSMGGAVASITSGRNPLIKSCVLWSAVCHFDIFFNRSPEEVSRIKDYGDFIDLGGNPVGKKFLSEIVNIKPLEEIKKRSIPVLIIHGSGDMVVPIQHAYDYFNGLKDTHKVKLEIIEGADHTFNSIEWEEKVIEKTVNWFKETL
Dimitra Zarafeta et al. (2016)
β EstDZ3: A New Esterolytic Enzyme Exhibiting Remarkable Thermostability
Frontiers in Microbiology
Β· doi:10.3389/fmicb.2016.01779 β
Β· Activity - Classical Stability - Incubation
▼
Database ID
UniProt: A0A1S5RMF8 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (23 measurements)
23 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 23.8 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | n-Hexane | 30% | 100.0 | 25.8 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | n-Hexane | 10% | 100.0 | 51.2 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isooctane | 30% | 100.0 | 33.6 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isooctane | 10% | 100.0 | 53.6 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 30% | 100.0 | 29.5 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 10% | 100.0 | 109.6 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 1-Butanol | 30% | 100.0 | 8.3 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 1-Butanol | 10% | 100.0 | 50.4 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 10% | 100.0 | 2.8 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 30% | 100.0 | 28.1 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 10% | 100.0 | 109.6 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 10% | 100.0 | 114.1 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 26.5 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 93.0 | % | 25 mM Tris-HCl, 0.05% Triton X-100 | 8 | 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Stability - Incubation | Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 50% | 100.0 | 96.0 | % | Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100 | Incubation: 8, Assay: 8 | Incubation: Room temperature, Assay: 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Yes, "vigorously agitated during incubation" | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | n-Hexane | 50% | 100.0 | 69.0 | % | Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100 | Incubation: 8, Assay: 8 | Incubation: Room temperature, Assay: 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Yes, "vigorously agitated during incubation" | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isooctane | 50% | 100.0 | 74.0 | % | Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100 | Incubation: 8, Assay: 8 | Incubation: Room temperature, Assay: 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Yes, "vigorously agitated during incubation" | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 50% | 100.0 | 59.0 | % | Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100 | Incubation: 8, Assay: 8 | Incubation: Room temperature, Assay: 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Yes, "vigorously agitated during incubation" | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
| Stability - Incubation | Activity after incubation (12 hours at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | 1-Butanol | 50% | 100.0 | 72.0 | % | Incubation: 25 mM Tris-HCl, 0.05% Triton X-100, Assay: 25 mM Tris-HCl, 0.05% Triton X-100 | Incubation: 8, Assay: 8 | Incubation: Room temperature, Assay: 75Β°C | 2 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | Yes, "vigorously agitated during incubation" | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.