Tannase (gene tanA) from Staphylococcus lugdunensis MTCC 3614

Enzyme Description

Species

Extremophile

EC Number

3.1.1.20 ↗

Sequence

Length: 613 amino acids

MKKTFISLLSATVILSGCGVGEHQNNNSNHDAKGVNTSNVKIKNYNQASSALQIDNSKWKYDSKNNVYYQLNISYVSNPQAKNVEKLGIYVPAAYFKGKKNHNGTYTVTVNDAKKVNGYSARTAPIVYPVNTPGYAEQSAPTSYRYSNISKYMKAGFIYVEAGLRGRSMSMGNNSSNASTKSYETGSPWGVTDLKAAIRYYRFNDSSLPGNSSKIYTFGHSGGGAQSAIAGASGDSKLYYKYLEQIGAAMTDKNGKYISDKIDGAMAWCPITSLDQADAAYEWQMGQYGNEGNRKKNSFQKQLSTDLASSYASYLNKLNLKNGNTTLSLTKSKNGQYTEGSYAKYLKKEIEDSATEFLNNTTFPYKQNSTEQAGMGNGGPSGGKPSGKMGSMPQMRKQSSNKTYKTMDAYLKDLNKKGTWITYDKKTKRAHITSLKDFAKYYKQPSKSVSAFDDLKRSQAENEVFGTSGSDSKLHFDQSLAKLLTENKSNYSKLNGWNSNYVSSYKNDLTKTDKLGTSMSTRMNMYNPMYYLSDYYSGYGKSNVANHWRIRTGIQQGDTALNTETNLSLALKERVGSKNVDFKTVWDQGHTMAETSGNSDSNFIKWVESINKK

Amballa Chaitanyakumar et al. (2016) — Expression, purification and immobilization of tannase from Staphylococcus lugdunensis MTCC 3614

AMB Express · doi:10.1186/s13568-016-0261-5 ↗ · Stability - Incubation

21 measurements ▼

Database ID

UniProt: Q0KKP0 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (21 measurements)

21 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Methanol	40%	100	5	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid , Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Isoamyl Alcohol	40%	100	61	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Benzene	60%	100	90	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Benzene	40%	100	103	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Benzene	20%	100	114	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	1-Butanol	60%	100	49	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	1-Butanol	40%	100	74	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	1-Butanol	20%	100	84	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Toluene	60%	100	110	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Toluene	40%	100	123	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Toluene	20%	100	151	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Isoamyl Alcohol	60%	100	53	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Methanol	20%	100	45	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Isoamyl Alcohol	20%	100	65	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	n-Hexane	60%	100	85	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	n-Hexane	40%	100	106	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	n-Hexane	20%	100	113	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	60%	100	30	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	40%	100	40	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100	64	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Methanol	60%	100	0	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (chromogenic assay, gallic acid and rodamine reaction product absorbance measurement, 520 nm) in aqueous phase	Methanol	40%	100	5	%	Incubation: 50 mM sodium phosphate, Assay: 17 mM sodium phosphate	Incubation: 8, Assay: 8	Incubation: Room temperature, Assay: 37°C	7 mM Methyl gallate	Gallic Acid, Methanol	—	—	Non-incubated control (in %), assay measured in aqueous phase \| Uncertainty about whether assay was in the presence of organic solvent or in aqueous phase

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Visualization : Stability — Incubation

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