Lipase (gene LipAMS8) from Pseudomonas sp. AMS8

Enzyme Description

Species
Pseudomonas sp. AMS8 β†—
Extremophile
Yes psychrophilic organism
EC Number
3.1.1.3 β†—

Sequence

Length: 476 amino acids
MGVFDYKNLGTEGSKALFADAMAITLYSYHNLDNGFAVGYQHNGFGLGLPATLVGALLGSTDSQGVIPGIPWNPDSEKAALEAVNKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQVEVLGKYDGDGKLLEIGIGFRGTSGPRETLITDSIGDLVSDLLAALGPKDYAKNYAGEAFGTLLKDVAAYAGSHGLTGKDVVVSGHSLGGLAVNSMADLSGNKWSGFYKDSNYVAYASPTQSSGDKVLNIGYENDPVFRALDGSSFNFSSLGVHDKPHESTTDNIVSFNDHYASTLWNVLPFSIVNVPTWLSHLPTGYGDGLTRVLDSKFYDLTSRDSTIIVANLSDPARANTWVQDLNRNAEPHKGNTFIIGSDGNDLIQGGKGVDFIEGGKGNDTIRDNSGHNTFLFGGQFGQDRVIGYQSTDKLVFKDVEGSADWRDHAKVVGGDTVLSFGADSVTLVGVGLAGVGGDGISIS
Menega Ganasen et al. (2016) β€” Cold-adapted organic solvent tolerant alkalophilic family I.3 lipase from an Antarctic Pseudomonas
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2016.06.095 β†—  Β· Activity - Michaelis-Menten Stability - Incubation
43 measurements ▼
Database ID
UniProt: E1B2U7 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (43 measurements)

43 measurements β€” page 2 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Michaelis-Menten kcat/kM (nM^-1 * s^-1) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Toluene ?% 0.6 0.4 nM^-1 * s^-1 Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nM^-1 * s^-1)
Activity - Michaelis-Menten kcat/kM (nM^-1 * s^-1) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent n-Hexane ?% 0.6 0.3 nM^-1 * s^-1 Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nM^-1 * s^-1)
Activity - Michaelis-Menten Vmax (V) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Methanol ?% 5.1 5.2 V Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in V)
Activity - Michaelis-Menten Vmax (V) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) ?% 5.1 9.9 V Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in V)
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Ethanol 25% 100.0 88.3 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 100.0 122.3 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Methanol 25% 100.0 101.9 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Pyridine 25% 100.0 0.0 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase 1-Butanol 25% 100.0 0.2 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Isoamyl Alcohol 25% 100.0 0.9 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Propyl Acetate 25% 100.0 7.0 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase 1-Propanol 25% 100.0 4.7 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Benzene 25% 100.0 66.1 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Toluene 25% 100.0 78.1 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Xylene 25% 100.0 91.9 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase n-Hexane 25% 100.0 101.4 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase 1-Decanol 25% 100.0 24.4 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase Isooctane 25% 100.0 118.4 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase n-Octane 25% 100.0 108.8 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 15Β°C), measured by absorbance spectrophotometry (Kwon and Rhee colorimetric assay, absorbance of copper acetate–pyridine and free fatty acids reaction product measurement, 715 nm) in aqueous phase n-Decane 25% 100.0 126.1 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 15Β°C, Assay: 30Β°C 35% Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay measured in aqueous phase | High uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
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Visualization : Activity β€” Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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