Lipase (gene LipAMS8) from Pseudomonas sp. AMS8

Enzyme Description

Species
Pseudomonas sp. AMS8 β†—
Extremophile
Yes psychrophilic organism
EC Number
3.1.1.3 β†—

Sequence

Length: 476 amino acids
MGVFDYKNLGTEGSKALFADAMAITLYSYHNLDNGFAVGYQHNGFGLGLPATLVGALLGSTDSQGVIPGIPWNPDSEKAALEAVNKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQVEVLGKYDGDGKLLEIGIGFRGTSGPRETLITDSIGDLVSDLLAALGPKDYAKNYAGEAFGTLLKDVAAYAGSHGLTGKDVVVSGHSLGGLAVNSMADLSGNKWSGFYKDSNYVAYASPTQSSGDKVLNIGYENDPVFRALDGSSFNFSSLGVHDKPHESTTDNIVSFNDHYASTLWNVLPFSIVNVPTWLSHLPTGYGDGLTRVLDSKFYDLTSRDSTIIVANLSDPARANTWVQDLNRNAEPHKGNTFIIGSDGNDLIQGGKGVDFIEGGKGNDTIRDNSGHNTFLFGGQFGQDRVIGYQSTDKLVFKDVEGSADWRDHAKVVGGDTVLSFGADSVTLVGVGLAGVGGDGISIS
Menega Ganasen et al. (2016) β€” Cold-adapted organic solvent tolerant alkalophilic family I.3 lipase from an Antarctic Pseudomonas
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2016.06.095 β†—  Β· Activity - Michaelis-Menten Stability - Incubation
43 measurements ▼
Database ID
UniProt: E1B2U7 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (43 measurements)

43 measurements β€” page 1 of 3
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Michaelis-Menten kcat (in 1/s) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Methanol ?% 135.8 115.5 1/s Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in 1/s)
Activity - Michaelis-Menten Vmax (V) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Isopropanol ?% 5.1 37.2 V Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in V)
Activity - Michaelis-Menten Vmax (V) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Toluene ?% 5.1 2.9 V Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in V)
Activity - Michaelis-Menten Vmax (V) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent n-Hexane ?% 5.1 4.7 V Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in V)
Activity - Michaelis-Menten Km (in nm) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) ?% 216.5 286.4 nm Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nm)
Activity - Michaelis-Menten Km (in nm) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Methanol ?% 216.5 189.5 nm Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nm)
Activity - Michaelis-Menten Km (in nm) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Ethanol ?% 216.5 650.6 nm Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nm)
Activity - Michaelis-Menten Km (in nm) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Isopropanol ?% 216.5 573.2 nm Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nm)
Activity - Michaelis-Menten Km (in nm) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Toluene ?% 216.5 194.6 nm Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nm)
Activity - Michaelis-Menten Km (in nm) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent n-Hexane ?% 216.5 278.2 nm Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nm)
Activity - Michaelis-Menten kcat (in 1/s) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) ?% 135.8 172.9 1/s Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in 1/s)
Activity - Michaelis-Menten Vmax (V) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Ethanol ?% 5.1 47.4 V Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in V)
Activity - Michaelis-Menten kcat (in 1/s) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Ethanol ?% 135.8 738.9 1/s Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in 1/s)
Activity - Michaelis-Menten kcat (in 1/s) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Isopropanol ?% 135.8 541.7 1/s Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in 1/s)
Activity - Michaelis-Menten kcat (in 1/s) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Toluene ?% 135.8 84.1 1/s Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in 1/s)
Activity - Michaelis-Menten kcat (in 1/s) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent n-Hexane ?% 135.8 100.2 1/s Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in 1/s)
Activity - Michaelis-Menten kcat/kM (nM^-1 * s^-1) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) ?% 0.6 0.6 nM^-1 * s^-1 Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nM^-1 * s^-1)
Activity - Michaelis-Menten kcat/kM (nM^-1 * s^-1) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Methanol ?% 0.6 0.6 nM^-1 * s^-1 Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nM^-1 * s^-1)
Activity - Michaelis-Menten kcat/kM (nM^-1 * s^-1) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Ethanol ?% 0.6 1.1 nM^-1 * s^-1 Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nM^-1 * s^-1)
Activity - Michaelis-Menten kcat/kM (nM^-1 * s^-1) determined by p-nitrophenol absorbance measurement (405 nm) in the presence of organic solvent Isopropanol ?% 0.6 0.9 nM^-1 * s^-1 Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer 7 25Β°C p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in nM^-1 * s^-1)
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Visualization : Activity β€” Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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