Esterase (gene EstSL3) from Alkalibacterium sp. SL3

Enzyme Description

Species

Alkalibacterium sp. SL3 ↗

Extremophile

Yes "halophilic", "alkalophilic" organism,"cold-adapted" enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 211 amino acids

MKINKNDTLLFIGDSITDVGRDRMDGEDLGKGFPLMVASHLQSRYPAKRLTVLNRGIGGDSLKDLKRRWEDDCLITNPDIVTLLIGVNDTWRNQNDGVELTDEELDEFESDYRFLLKSLHQRTDARVILMESFVLPYPKRRVGWRNDLDKRIQIVRKMARDYQTELIPLDGLLNAAGIRDGFSYYTGDDGVHPTVAGHGLIANSWLKAVDE

Guozeng Wang et al. (2016) — A novel cold-adapted and highly salt-tolerant esterase from Alkalibacterium sp. SL3 from the sediment of a soda lake

Scientific Reports · doi:10.1038/srep19494 ↗ · Activity - Classical

22 measurements ▼

Database ID

UniProt: A0A140IHU5 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 2 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	93.9	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100.0	72.7	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isoamyl Alcohol	20%	100.0	7.3	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	74.2	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	92.7	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Chloroform	20%	100.0	45.6	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Chloroform	10%	100.0	57.1	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Glycerol	20%	100.0	81.9	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Glycerol	10%	100.0	77.5	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	20%	100.0	89.1	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	n-Hexane	10%	100.0	98.1	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	100.0	24.4	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100.0	48.4	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100.0	75.8	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isoamyl Alcohol	10%	100.0	34.8	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isobutanol	20%	100.0	33.0	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isobutanol	10%	100.0	73.5	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	20%	100.0	24.3	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Butanol	10%	100.0	38.4	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Propanol	20%	100.0	91.2	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	1-Propanol	10%	100.0	97.9	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100.0	87.4	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	93.9	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100.0	72.7	%	50 mM Tris–HCl buffer, 10% acetonitrile	9	30°C	1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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Structure

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