Cytochrome c from Equus caballus (horse)

Enzyme Description

Extremophile
No
EC Number

Sequence

Length: 105 amino acids
MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGFTYTDANKNKGITWKEETLMEYLENPKKYIPGTKMIFAGIKKKTEREDLIAYLKKATNE
Rafael Vazquez-Duhalt et al. (1993) β€” Effect of water-miscible organic solvents on the catalytic activity of cytochrome c
Enzyme and Microbial Technology  Β· doi:10.1016/0141-0229(93)90169-3 β†—  Β· Activity - Classical Activity - Michaelis-Menten
72 measurements
Database ID
UniProt: P00004 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Commercially purchased

Experimental Data (72 measurements)

72 measurements β€” page 4 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Michaelis-Menten Km (mM) Tetrahydrofuran (THF) 60% 21.5 1.3 mM 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten Km (mM) Tetrahydrofuran (THF) 70% 21.5 0.7 mM 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten Km (mM) Tetrahydrofuran (THF) 80% 21.5 11.8 mM 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten Km (mM) Tetrahydrofuran (THF) 90% 21.5 10.8 mM 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in mM) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten kcat/Km Tetrahydrofuran (THF) 20% 0.72 0.88 deltaA603/(min*nmol*mM) 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten kcat/Km Tetrahydrofuran (THF) 30% 0.72 1.05 deltaA603/(min*nmol*mM) 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten kcat/Km Tetrahydrofuran (THF) 40% 0.72 1.6 deltaA603/(min*nmol*mM) 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten kcat/Km Tetrahydrofuran (THF) 50% 0.72 1.71 deltaA603/(min*nmol*mM) 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten kcat/Km Tetrahydrofuran (THF) 60% 0.72 1.46 deltaA603/(min*nmol*mM) 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten kcat/Km Tetrahydrofuran (THF) 70% 0.72 0.39 deltaA603/(min*nmol*mM) 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten kcat/Km Tetrahydrofuran (THF) 80% 0.72 0.22 deltaA603/(min*nmol*mM) 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
Activity - Michaelis-Menten kcat/Km Tetrahydrofuran (THF) 90% 0.72 0.34 deltaA603/(min*nmol*mM) 6 mM phosphate buffer 6.1 Room temperature Hβ‚‚Oβ‚‚ (Hydrogen Peroxide), Pinacyanol chloride H2O , Oxidized pinacyanol β€” β€” Classical aqueous control (in deltaA603/(min*nmol*mM)) but in the presence of 10% THF | Assay-specific EC number (enzyme peroxidase activity)/Control in the presence of low volume of organic solvent
1 2 3 4
Next β€Ί

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity β€” Michaelis-Menten

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*